Functional Role of the Prokaryotic Proline-tRNA Synthetase Insertion Domain in Amino Acid Editing

• Published in: Biochemistry (Easton) Vol. 41; no. 22; pp. 7108 - 7115
• Main Authors: Wong, Fai-Chu, Beuning, Penny J, Nagan, Maria, Shiba, Kiyotaka, Musier-Forsyth, Karin
• Format: Journal Article
• Language: English
• Published: United States American Chemical Society 04.06.2002
• Subjects: Alanine - genetics; Alanine - metabolism; Amino Acyl-tRNA Synthetases - chemistry; Amino Acyl-tRNA Synthetases - metabolism; Binding Sites - physiology; Molecular Sequence Data; Mutagenesis, Insertional - physiology; Prokaryotic Cells - enzymology; Proline - metabolism; Protein Structure, Tertiary - physiology; RNA Editing - physiology; Sequence Alignment

Aminoacyl-tRNA synthetases catalyze the attachment of specific amino acids to cognate tRNAs in a two-step process that is critical for the faithful translation of genetic information. During the first chemical step of tRNA aminoacylation, noncognate amino acids that are smaller than or isosteric wit...