Intron Retained Bioluminescence Reporter for Real-Time Imaging of Pre-mRNA Splicing in Living Subjects

• Published in: Analytical chemistry (Washington) Vol. 91; no. 19; pp. 12392 - 12398
• Main Authors: Zheng, Haifeng, Chen, Si, Wang, Xinan, Xie, Jinrong, Tian, Jie, Wang, Fu
• Format: Journal Article
• Language: English
• Published: United States American Chemical Society 01.10.2019
• Subjects: Bioluminescence; Cells (biology); Deoxyribonucleic acid; DNA; Eukaryotes; eukaryotic cells; Genetic code; human diseases; image analysis; information exchange; introns; luciferase; messenger RNA; mice; Modulators; Monitoring; mRNA; Nucleotide sequence; Pharmacogenomics; Real time; Renilla; RNA splicing; Splicing; therapeutics

Pre-mRNA splicing in the information exchange from DNA to protein is a critical step in all eukaryotes. However, there is currently a lack of noninvasive approaches for monitoring mRNA splicing events in cells. In this study, we presented a genetically encoded bioluminescence reporter, Rluc-intron,...