Tandem Action of Glycosyltransferases in the Maturation of Vancomycin and Teicoplanin Aglycones:  Novel Glycopeptides

• Published in: Biochemistry (Easton) Vol. 40; no. 15; pp. 4745 - 4755
• Main Authors: Losey, Heather C, Peczuh, Mark W, Chen, Zhong, Eggert, Ulrike S, Dong, Steven D, Pelczer, Istvan, Kahne, Daniel, Walsh, Christopher T
• Format: Journal Article
• Language: English
• Published: United States American Chemical Society 17.04.2001
• Subjects: Actinomycetales - enzymology; Amycolatopsis orientalis; Anti-Bacterial Agents - biosynthesis; Escherichia coli; Glucosyltransferases - isolation & purification; Glucosyltransferases - metabolism; glycosyltransferase; Hexosamines - chemical synthesis; Hexosamines - metabolism; Microbial Sensitivity Tests; Molecular Sequence Data; Substrate Specificity; Teicoplanin - biosynthesis; Teicoplanin - chemistry; Uridine Diphosphate Glucose - metabolism; Vancomycin - analogs & derivatives; Vancomycin - biosynthesis; Vancomycin - chemistry

The glycopeptides vancomycin and teicoplanin are clinically important antibiotics. The carbohydrate portions of these molecules affect biological activity, and there is great interest in developing efficient strategies to make carbohydrate derivatives. To this end, genes encoding four glycosyltransf...