Targeted Proteomic Analysis of Glycolysis in Cancer Cells

• Published in: Journal of proteome research Vol. 10; no. 2; pp. 604 - 613
• Main Authors: Murphy, J. Patrick, Pinto, Devanand M
• Format: Journal Article
• Language: English
• Published: United States American Chemical Society 04.02.2011
• Subjects: Cell Line, Tumor; Computer Simulation; Female; Glycolysis; Humans; Insulin-Like Growth Factor I - metabolism; Isotope Labeling; Mass Spectrometry; Neoplasms - enzymology; Neoplasms - metabolism; Proteome - analysis; Proteome - metabolism; Proteomics; Sequence Analysis, Protein; lactic acid; invasiveness; multiple reaction monitoring; glycolysis; pyruvate kinase M2; targeted proteomics; Warburg effect

Altered expression of glycolysis proteins is an important yet poorly understood characteristic of cancer. To better understand the glycolytic changes during tumorigenesis, we designed a liquid chromatography multiple reaction monitoring (LC−MRM) assay targeting the “glycolysis proteome” in MCF-7 bre...