Impact of HIV‑1 Membrane Cholesterol on Cell-Independent Lytic Inactivation and Cellular Infectivity

• Published in: Biochemistry (Easton) Vol. 55; no. 3; pp. 447 - 458
• Main Authors: Kalyana Sundaram, Ramalingam Venkat, Li, Huiyuan, Bailey, Lauren, Rashad, Adel A, Aneja, Rachna, Weiss, Karl, Huynh, James, Bastian, Arangaserry Rosemary, Papazoglou, Elisabeth, Abrams, Cameron, Wrenn, Steven, Chaiken, Irwin
• Format: Journal Article
• Language: English
• Published: United States American Chemical Society 26.01.2016
• Subjects: beta-Cyclodextrins - pharmacology; Cell Line, Tumor; Cholesterol - metabolism; HIV Core Protein p24 - metabolism; HIV Envelope Protein gp120 - metabolism; HIV-1 - drug effects; HIV-1 - pathogenicity; HIV-1 - physiology; Humans; Membrane Fluidity; Membrane Lipids - metabolism; Peptides - chemistry; Peptides - pharmacology; Sulfhydryl Compounds - chemistry; Sulfhydryl Compounds - pharmacology; Thiazoles - chemistry; Thiazoles - pharmacology; Triazoles - chemistry; Triazoles - pharmacology

Peptide triazole thiols (PTTs) have been found previously to bind to HIV-1 Env spike gp120 and cause irreversible virus inactivation by shedding gp120 and lytically releasing luminal capsid protein p24. Since the virions remain visually intact, lysis appears to occur via limited membrane destabiliza...