Kinetics of Ribosome-Catalyzed Polymerization Using Artificial Aminoacyl-tRNA Substrates Clarifies Inefficiencies and Improvements

• Published in: ACS chemical biology Vol. 10; no. 10; pp. 2187 - 2192
• Main Authors: Wang, Jinfan, Kwiatkowski, Marek, Forster, Anthony C
• Format: Journal Article
• Language: English
• Published: United States American Chemical Society 16.10.2015
• Subjects: Biochemistry; Biokemi; Catalysis; Kinetics; Models, Biological; Peptides - chemical synthesis; Peptides - chemistry; Peptides - metabolism; Polymerization; Ribosomes - metabolism; RNA, Transfer, Amino Acyl - chemistry

Ribosomal synthesis of polymers of unnatural amino acids (AAs) is limited by low incorporation efficiencies using the artificial AA-tRNAs, but the kinetics have yet to be studied. Here, kinetics were performed on five consecutive incorporations using various artificial AA-tRNAs with all intermediate...