Chromatographic Determination of Cyanoglycosides Prunasin and Amygdalin in Plant Extracts Using a Porous Graphitic Carbon Column
The determination of cyanogenic compounds in plants is often performed by HPLC. However, in this analysis, interferences due to compounds in the matrix, such as tannins and other pigments, are encountered, especially in roots and leaves. A new method is proposed for determining the cyanogenic glycos...
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Published in | Journal of agricultural and food chemistry Vol. 50; no. 24; pp. 6960 - 6963 |
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Main Authors | , , , |
Format | Journal Article |
Language | English |
Published |
Washington, DC
American Chemical Society
20.11.2002
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Subjects | |
Online Access | Get full text |
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Summary: | The determination of cyanogenic compounds in plants is often performed by HPLC. However, in this analysis, interferences due to compounds in the matrix, such as tannins and other pigments, are encountered, especially in roots and leaves. A new method is proposed for determining the cyanogenic glycosides amygdalin (d-mandelonitrile β-d-gentiobioside) and prunasin (d-mandelonitrile β-d-glucoside) in almond tree tissues, using poly(vinylpyrrolidone) or active carbon as scavengers for extracting cyanogenic compounds from roots or leaves, respectively. A new chromatographic approach for conducting the analysis is also discussed herein. The advantages of a Hypercarb column for the analysis of prunasin in roots are shown. The correlation coefficient with a reference method is high (>0.99), and statistical tests prove that the two methods are equivalent. In addition, the results provide evidence that prunasin is the only cyanoglycoside present in almond tree roots. Keywords: Chromatography; amygdalin; prunasin; almond tree tissues |
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Bibliography: | istex:793F1A849242C3C1538691B2CF5219A197A40334 ark:/67375/TPS-W1T689BG-5 ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
ISSN: | 0021-8561 1520-5118 |
DOI: | 10.1021/jf0256081 |