Does the Reconstitution of RC-LH1 Complexes from Rhodopseudomonas acidophila Strain 10050 into a Phospholipid Bilayer Yield the Optimum Environment for Optical Spectroscopy?

• Published in: The journal of physical chemistry. B Vol. 117; no. 48; pp. 15004 - 15013
• Main Authors: Böhm, Paul S, Kunz, Ralf, Southall, June, Cogdell, Richard J, Köhler, Jürgen
• Format: Journal Article
• Language: English
• Published: Washington, DC American Chemical Society 05.12.2013
• Subjects: Biological and medical sciences; Buffer solutions; Detergents; Fundamental and applied biological sciences. Psychology; Immobilization; Light-Harvesting Protein Complexes - chemistry; Light-Harvesting Protein Complexes - metabolism; Lipid Bilayers - chemistry; Lipid Bilayers - metabolism; Models, Molecular; Molecular biophysics; Phospholipids; Phospholipids - chemistry; Phospholipids - metabolism; physical chemistry; polyvinyl alcohol; Polyvinyl alcohols; Proteins; Radiation-biomolecule interaction; Rhodopseudomonas; Rhodopseudomonas - chemistry; Rhodopseudomonas - enzymology; Rhodopseudomonas acidophila; Spectra; Spectrophotometry, Ultraviolet; Spectroscopy; Spectroscopy, Near-Infrared; statistics; Rhodospirillales; Light harvesting system; Medium effect; Phospholipid; Dipolar transition; Bacteria; Rhodospirillaceae; Rhodopseudomonas acidophila; Rhodospirillineae; Protein; Absorption spectrum; Bilayers

We have investigated reaction-center light-harvesting 1 (RC-LH1) complexes from Rhodopseudomonas (Rps.) acidophila in detergent buffer solution and reconstituted into a phospholipid bilayer and compared the results with the outcome of an earlier study conducted on RC-LH1 immobilized in polyvinyl alc...