Simultaneous Electrochemical Analysis of Hydrophilic and Lipophilic Antioxidants in Bicontinuous Microemulsion
Qualitative and quantitative analyses of hydrophilic and lipophilic antioxidants, such as polyphenols, by simple electrochemical measurements were conducted in a bicontinuous microemulsion (BME), in which water and oil phases coexisted bicontinuously on a microscopic scale. Hydrophilic and lipophili...
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| Published in | Analytical chemistry (Washington) Vol. 87; no. 3; pp. 1489 - 1493 |
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| Main Authors | , , , , , , |
| Format | Journal Article |
| Language | English |
| Published |
United States
American Chemical Society
03.02.2015
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| Abstract | Qualitative and quantitative analyses of hydrophilic and lipophilic antioxidants, such as polyphenols, by simple electrochemical measurements were conducted in a bicontinuous microemulsion (BME), in which water and oil phases coexisted bicontinuously on a microscopic scale. Hydrophilic and lipophilic antioxidants were individually monitored in the same BME solution using a hydrophilic indium tin oxide (ITO) electrode and a lipophilic fluorinated nanocarbon film electrode (F-ECR), respectively. The combination of well-balanced BME and extremely biased electrodes, such as ITO and F-ECR, in terms of hydrophilic–lipophilic balance allowed us to achieve individual monitoring of hydrophilic and lipophilic antioxidants in the same BME solution without extraction. Furthermore, the antioxidant activities of functional liquid foods, such as coffee and olive oil, were also evaluated by means of electrochemical measurements in BME solutions containing analytes in concentrations of several percent. The technique we propose provides a very simple, rapid, easily serviceable, and highly reproducible analysis and can be extended to a wide range of analytes and media. |
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| AbstractList | Qualitative and quantitative analyses of hydrophilic and lipophilic antioxidants, such as polyphenols, by simple electrochemical measurements were conducted in a bicontinuous microemulsion (BME), in which water and oil phases coexisted bicontinuously on a microscopic scale. Hydrophilic and lipophilic antioxidants were individually monitored in the same BME solution using a hydrophilic indium tin oxide (ITO) electrode and a lipophilic fluorinated nanocarbon film electrode (F-ECR), respectively. The combination of well-balanced BME and extremely biased electrodes, such as ITO and F-ECR, in terms of hydrophilic–lipophilic balance allowed us to achieve individual monitoring of hydrophilic and lipophilic antioxidants in the same BME solution without extraction. Furthermore, the antioxidant activities of functional liquid foods, such as coffee and olive oil, were also evaluated by means of electrochemical measurements in BME solutions containing analytes in concentrations of several percent. The technique we propose provides a very simple, rapid, easily serviceable, and highly reproducible analysis and can be extended to a wide range of analytes and media. |
| Author | Nagatomo, Shota Kato, Dai Nishimi, Taisei Sakata, Kouhei Niwa, Osamu Kuraya, Eisuke Kunitake, Masashi |
| AuthorAffiliation | Graduate School of Science and Technology Fujifilm Corporation Okinawa National College of Technology National Institute of Advanced Industrial Science and Technology (AIST) Science and Technology Division Kumamoto University |
| AuthorAffiliation_xml | – name: Okinawa National College of Technology – name: Graduate School of Science and Technology – name: Science and Technology Division – name: National Institute of Advanced Industrial Science and Technology (AIST) – name: Kumamoto University – name: Fujifilm Corporation |
| Author_xml | – sequence: 1 givenname: Eisuke surname: Kuraya fullname: Kuraya, Eisuke organization: Kumamoto University – sequence: 2 givenname: Shota surname: Nagatomo fullname: Nagatomo, Shota organization: Kumamoto University – sequence: 3 givenname: Kouhei surname: Sakata fullname: Sakata, Kouhei organization: Kumamoto University – sequence: 4 givenname: Dai surname: Kato fullname: Kato, Dai organization: National Institute of Advanced Industrial Science and Technology (AIST) – sequence: 5 givenname: Osamu surname: Niwa fullname: Niwa, Osamu organization: National Institute of Advanced Industrial Science and Technology (AIST) – sequence: 6 givenname: Taisei surname: Nishimi fullname: Nishimi, Taisei organization: Fujifilm Corporation – sequence: 7 givenname: Masashi surname: Kunitake fullname: Kunitake, Masashi email: kunitake@kumamoto-u.ac.jp organization: Kumamoto University |
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| SubjectTerms | alpha-Tocopherol - chemistry Analytical chemistry Antioxidants Antioxidants - chemistry Ascorbic Acid - chemistry Biochemistry Coffee - chemistry Electrochemical Techniques Electrodes Emulsions - chemistry Extraction Food Analysis Hydrophobic and Hydrophilic Interactions Indium tin oxide Lipids - chemistry Metal oxides Microemulsions Nanostructure Olive Oil Oxidation-Reduction Plant Oils - chemistry Polyphenols Quantitative analysis Tin Compounds - chemistry |
| Title | Simultaneous Electrochemical Analysis of Hydrophilic and Lipophilic Antioxidants in Bicontinuous Microemulsion |
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