Synchrotron FTIR Microspectroscopy of Single Natural Silk Fibers

Synchrotron FTIR (S-FTIR) microspectroscopy was used to monitor the silk protein conformation in a range of single natural silk fibers (domestic and wild silkworm and spider dragline silk). With the selection of suitable aperture size, we obtained high-resolution S-FTIR spectra capable of semiquanti...

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Published in	Biomacromolecules Vol. 12; no. 9; pp. 3344 - 3349
Main Authors	Ling, Shengjie, Qi, Zeming, Knight, David P, Shao, Zhengzhong, Chen, Xin
Format	Journal Article
Language	English
Published	Washington, DC American Chemical Society 12.09.2011
Subjects	Animals Antheraea pernyi Applied sciences Araneae Biocompatible Materials - analysis Biocompatible Materials - chemistry Bombyx - chemistry Bombyx mori Exact sciences and technology Fibers and threads Fibroins - analysis Fibroins - chemistry Forms of application and semi-finished materials Magnetic Resonance Spectroscopy Nephila edulis Polymer industry, paints, wood Protein Structure, Secondary Silk - analysis Silk - chemistry Spectroscopy, Fourier Transform Infrared - methods Spectrum Analysis, Raman Spiders - chemistry Synchrotrons Technology of polymers Fourier transformation Saturniidae Insecta Bombyx mori Experimental study Infrared spectrometry Solid state Silk Arthropoda Natural fiber Bombycidae Lepidoptera Antheraea pernyi Invertebrata Comparative study Synchrotron radiation Conformation
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Abstract	Synchrotron FTIR (S-FTIR) microspectroscopy was used to monitor the silk protein conformation in a range of single natural silk fibers (domestic and wild silkworm and spider dragline silk). With the selection of suitable aperture size, we obtained high-resolution S-FTIR spectra capable of semiquantitative analysis of protein secondary structures. For the first time, we have determined from S-FTIR the β-sheet content in a range of natural single silk fibers, 28 ± 4, 23 ± 2, and 17 ± 4% in Bombyx mori, Antheraea pernyi, and Nephila edulis silks, respectively. The trend of β-sheet content in different silk fibers from the current study accords quite well with published data determined by XRD, Raman, and 13C NMR. Our results indicate that the S-FTIR microspectroscopy method has considerable potential for the study of single natural silk fibers.
AbstractList	Synchrotron FTIR (S-FTIR) microspectroscopy was used to monitor the silk protein conformation in a range of single natural silk fibers (domestic and wild silkworm and spider dragline silk). With the selection of suitable aperture size, we obtained high-resolution S-FTIR spectra capable of semiquantitative analysis of protein secondary structures. For the first time, we have determined from S-FTIR the β-sheet content in a range of natural single silk fibers, 28 ± 4, 23 ± 2, and 17 ± 4% in Bombyx mori, Antheraea pernyi, and Nephila edulis silks, respectively. The trend of β-sheet content in different silk fibers from the current study accords quite well with published data determined by XRD, Raman, and (13)C NMR. Our results indicate that the S-FTIR microspectroscopy method has considerable potential for the study of single natural silk fibers.Synchrotron FTIR (S-FTIR) microspectroscopy was used to monitor the silk protein conformation in a range of single natural silk fibers (domestic and wild silkworm and spider dragline silk). With the selection of suitable aperture size, we obtained high-resolution S-FTIR spectra capable of semiquantitative analysis of protein secondary structures. For the first time, we have determined from S-FTIR the β-sheet content in a range of natural single silk fibers, 28 ± 4, 23 ± 2, and 17 ± 4% in Bombyx mori, Antheraea pernyi, and Nephila edulis silks, respectively. The trend of β-sheet content in different silk fibers from the current study accords quite well with published data determined by XRD, Raman, and (13)C NMR. Our results indicate that the S-FTIR microspectroscopy method has considerable potential for the study of single natural silk fibers. Synchrotron FTIR (S-FTIR) microspectroscopy was used to monitor the silk protein conformation in a range of single natural silk fibers (domestic and wild silkworm and spider dragline silk). With the selection of suitable aperture size, we obtained high-resolution S-FTIR spectra capable of semiquantitative analysis of protein secondary structures. For the first time, we have determined from S-FTIR the β-sheet content in a range of natural single silk fibers, 28 ± 4, 23 ± 2, and 17 ± 4% in Bombyx mori, Antheraea pernyi, and Nephila edulis silks, respectively. The trend of β-sheet content in different silk fibers from the current study accords quite well with published data determined by XRD, Raman, and (13)C NMR. Our results indicate that the S-FTIR microspectroscopy method has considerable potential for the study of single natural silk fibers. Synchrotron FTIR (S-FTIR) microspectroscopy was used to monitor the silk protein conformation in a range of single natural silk fibers (domestic and wild silkworm and spider dragline silk). With the selection of suitable aperture size, we obtained high-resolution S-FTIR spectra capable of semiquantitative analysis of protein secondary structures. For the first time, we have determined from S-FTIR the β-sheet content in a range of natural single silk fibers, 28 ± 4, 23 ± 2, and 17 ± 4% in Bombyx mori, Antheraea pernyi, and Nephila edulis silks, respectively. The trend of β-sheet content in different silk fibers from the current study accords quite well with published data determined by XRD, Raman, and 13C NMR. Our results indicate that the S-FTIR microspectroscopy method has considerable potential for the study of single natural silk fibers. Synchrotron FTIR (S-FTIR) microspectroscopy was used to monitor the silk protein conformation in a range of single natural silk fibers (domestic and wild silkworm and spider dragline silk). With the selection of suitable aperture size, we obtained high-resolution S-FTIR spectra capable of semiquantitative analysis of protein secondary structures. For the first time, we have determined from S-FTIR the Delta b-sheet content in a range of natural single silk fibers, 28 ? 4, 23 ? 2, and 17 ? 4% in Bombyx mori, Antheraea pernyi, and Nephila edulis silks, respectively. The trend of Delta b-sheet content in different silk fibers from the current study accords quite well with published data determined by XRD, Raman, and 13C NMR. Our results indicate that the S-FTIR microspectroscopy method has considerable potential for the study of single natural silk fibers.
Author	Knight, David P Chen, Xin Ling, Shengjie Qi, Zeming Shao, Zhengzhong
AuthorAffiliation	University of Science and Technology of China Fudan University Oxford Biomaterials, Ltd
AuthorAffiliation_xml	– name: University of Science and Technology of China – name: Fudan University – name: Oxford Biomaterials, Ltd
Author_xml	– sequence: 1 givenname: Shengjie surname: Ling fullname: Ling, Shengjie – sequence: 2 givenname: Zeming surname: Qi fullname: Qi, Zeming – sequence: 3 givenname: David P surname: Knight fullname: Knight, David P – sequence: 4 givenname: Zhengzhong surname: Shao fullname: Shao, Zhengzhong – sequence: 5 givenname: Xin surname: Chen fullname: Chen, Xin email: chenx@fudan.edu.cn
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Keywords	Fourier transformation Saturniidae Insecta Bombyx mori Experimental study Infrared spectrometry Solid state Silk Arthropoda Natural fiber Bombycidae Lepidoptera Antheraea pernyi Invertebrata Comparative study Synchrotron radiation Conformation
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Snippet	Synchrotron FTIR (S-FTIR) microspectroscopy was used to monitor the silk protein conformation in a range of single natural silk fibers (domestic and wild...
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SubjectTerms	Animals Antheraea pernyi Applied sciences Araneae Biocompatible Materials - analysis Biocompatible Materials - chemistry Bombyx - chemistry Bombyx mori Exact sciences and technology Fibers and threads Fibroins - analysis Fibroins - chemistry Forms of application and semi-finished materials Magnetic Resonance Spectroscopy Nephila edulis Polymer industry, paints, wood Protein Structure, Secondary Silk - analysis Silk - chemistry Spectroscopy, Fourier Transform Infrared - methods Spectrum Analysis, Raman Spiders - chemistry Synchrotrons Technology of polymers
Title	Synchrotron FTIR Microspectroscopy of Single Natural Silk Fibers
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