Contact with the CsrA Core Is Required for Allosteric Inhibition by FliW in Bacillus subtilis

• Published in: Journal of bacteriology Vol. 203; no. 2; pp. 1 - 15
• Main Authors: Oshiro, Reid T, Dunn, Caroline M, Kearns, Daniel B
• Format: Journal Article
• Language: English
• Published: United States American Society for Microbiology 18.12.2020
• Subjects: Alleles; Allosteric properties; Amino Acid Sequence; Antagonism; Bacillus subtilis; Bacillus subtilis - drug effects; Bacillus subtilis - genetics; Bacillus subtilis - physiology; Bacterial Proteins - chemistry; Bacterial Proteins - genetics; Bacterial Proteins - pharmacology; Bacteriology; Charge reversal; Dimerization; Dimers; Domains; Flagella; Flagellin; Genomes; Homeostasis; Loss of Function Mutation - genetics; Phylogeny; Post-transcription; Protein Conformation; Proteins; Regulators; Repressor Proteins - antagonists & inhibitors; Repressor Proteins - chemistry; Repressor Proteins - genetics; Repressor Proteins - metabolism; Research Article; Residues; Ribonucleic acid; RNA; RNA, Bacterial - metabolism; RNA-binding protein; RNA-Binding Proteins - antagonists & inhibitors; RNA-Binding Proteins - chemistry; RNA-Binding Proteins - genetics; RNA-Binding Proteins - metabolism; Salts; Spotlight; flagella; motility; RsmA; FliW; CsrA

The RNA-binding protein CsrA is a posttranscriptional regulator encoded by genomes throughout the bacterial phylogeny. In the gammaproteobacteria, the activity of CsrA is inhibited by small RNAs that competitively sequester CsrA binding. In contrast, the firmicute encodes a protein inhibitor of CsrA...