Metabolic Labeling with an Alkyne-modified Isoprenoid Analog Facilitates Imaging and Quantification of the Prenylome in Cells

• Published in: ACS chemical biology Vol. 11; no. 10; pp. 2820 - 2828
• Main Authors: Palsuledesai, Charuta C, Ochocki, Joshua D, Kuhns, Michelle M, Wang, Yen-Chih, Warmka, Janel K, Chernick, Dustin S, Wattenberg, Elizabeth V, Li, Ling, Arriaga, Edgar A, Distefano, Mark D
• Format: Journal Article
• Language: English
• Published: United States American Chemical Society 21.10.2016
• Subjects: Alkynes - chemistry; Autophagy; Flow Cytometry; HeLa Cells; Humans; Protein Prenylation; Terpenes - chemistry

Protein prenylation is a post-translational modification that is responsible for membrane association and protein–protein interactions. The oncogenic protein Ras, which is prenylated, has been the subject of intense study in the past 20 years as a therapeutic target. Several studies have shown a cor...