Multiplex, Real-Time, Point-of-care RT-LAMP for SARS-CoV‑2 Detection Using the HFman Probe

• Published in: ACS sensors Vol. 7; no. 3; pp. 730 - 739
• Main Authors: Dong, Yajuan, Zhao, Yongjuan, Li, Shenwei, Wan, Zhenzhou, Lu, Renfei, Yang, Xianguang, Yu, Guoying, Reboud, Julien, Cooper, Jonathan M., Tian, Zhengan, Zhang, Chiyu
• Format: Journal Article
• Language: English
• Published: United States American Chemical Society 25.03.2022
• Subjects: COVID-19 - diagnosis; COVID-19 Testing; Humans; Molecular Diagnostic Techniques; Nucleic Acid Amplification Techniques - methods; Point-of-Care Systems; RNA, Viral - analysis; RNA, Viral - genetics; SARS-CoV-2 - genetics; Sensitivity and Specificity; HFman probe; high-fidelity DNA polymerase; multiplex LAMP; COVID-19/SARS-CoV-2; non-specific amplification; point-of-care testing (POCT)

Viral evolution impacts diagnostic test performance through the emergence of variants with sequences affecting the efficiency of primer binding. Such variants that evade detection by nucleic acid-based tests are subject to selective pressure, enabling them to spread more efficiently. Here, we report...