Chondroitin Sulfate-Based Biomineralizing Surface Hydrogels for Bone Tissue Engineering

Chondroitin sulfate (CS) is the major component of glycosaminoglycan in connective tissue. In this study, we fabricated methacrylated PEGDA/CS-based hydrogels with varying CS concentration (0, 1, 5, and 10%) and investigated them as biomineralizing three-dimensional scaffolds for charged ion binding...

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Published inACS applied materials & interfaces Vol. 9; no. 26; pp. 21639 - 21650
Main Authors Kim, Hwan D, Lee, Eunjee A, An, Young-Hyeon, Kim, Seunghyun L, Lee, Seunghun S, Yu, Seung Jung, Jang, Hae Lin, Nam, Ki Tae, Im, Sung Gap, Hwang, Nathaniel S
Format Journal Article
LanguageEnglish
Published United States American Chemical Society 05.07.2017
Subjects
Bone and Bones
Cell Differentiation
Cells, Cultured
Chondroitin Sulfates - chemistry
Humans
Hydrogels
Mesenchymal Stem Cells
Osteogenesis
Tissue Engineering
Tissue Scaffolds
bone regeneration
osteogenesis
calcium phosphate
hydrogel
chondroitin sulfate
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Abstract Chondroitin sulfate (CS) is the major component of glycosaminoglycan in connective tissue. In this study, we fabricated methacrylated PEGDA/CS-based hydrogels with varying CS concentration (0, 1, 5, and 10%) and investigated them as biomineralizing three-dimensional scaffolds for charged ion binding and depositions. Due to its negative charge from the sulfate group, CS exhibited an osteogenically favorable microenvironment by binding charged ions such as calcium and phosphate. Particularly, ion binding and distribution within negatively charged hydrogel was dependent on CS concentration. Furthermore, CS dependent biomineralizing microenvironment induced osteogenic differentiation of human tonsil-derived mesenchymal stem cells in vitro. Finally, when we transplanted PEGDA/CS-based hydrogel into a critical sized cranial defect model for 8 weeks, 10% CS hydrogel induced effective bone formation with highest bone mineral density. This PEGDA/CS-based biomineralizing hydrogel platform can be utilized for in situ bone formation in addition to being an investigational tool for in vivo bone mineralization and resorption mechanisms.
AbstractList Chondroitin sulfate (CS) is the major component of glycosaminoglycan in connective tissue. In this study, we fabricated methacrylated PEGDA/CS-based hydrogels with varying CS concentration (0, 1, 5, and 10%) and investigated them as biomineralizing three-dimensional scaffolds for charged ion binding and depositions. Due to its negative charge from the sulfate group, CS exhibited an osteogenically favorable microenvironment by binding charged ions such as calcium and phosphate. Particularly, ion binding and distribution within negatively charged hydrogel was dependent on CS concentration. Furthermore, CS dependent biomineralizing microenvironment induced osteogenic differentiation of human tonsil-derived mesenchymal stem cells in vitro. Finally, when we transplanted PEGDA/CS-based hydrogel into a critical sized cranial defect model for 8 weeks, 10% CS hydrogel induced effective bone formation with highest bone mineral density. This PEGDA/CS-based biomineralizing hydrogel platform can be utilized for in situ bone formation in addition to being an investigational tool for in vivo bone mineralization and resorption mechanisms.
Author Yu, Seung Jung
An, Young-Hyeon
Lee, Eunjee A
Kim, Hwan D
Lee, Seunghun S
Jang, Hae Lin
Nam, Ki Tae
Hwang, Nathaniel S
Im, Sung Gap
Kim, Seunghyun L
AuthorAffiliation Department of Chemical and Bimolecular Engineering
Interdisciplinary Program in Bioengineering
Department of Materials Science and Engineering
Korea Advanced Institute of Technology
Seoul National University
School of Chemical and Biological Engineering, N-Bio Institute, Institute of Chemical Process
AuthorAffiliation_xml – name: School of Chemical and Biological Engineering, N-Bio Institute, Institute of Chemical Process
– name: Department of Chemical and Bimolecular Engineering
– name: Korea Advanced Institute of Technology
– name: Seoul National University
– name: Interdisciplinary Program in Bioengineering
– name: Department of Materials Science and Engineering
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  organization: Seoul National University
BackLink https://www.ncbi.nlm.nih.gov/pubmed/28605908$$D View this record in MEDLINE/PubMed
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osteogenesis
calcium phosphate
hydrogel
chondroitin sulfate
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Snippet Chondroitin sulfate (CS) is the major component of glycosaminoglycan in connective tissue. In this study, we fabricated methacrylated PEGDA/CS-based hydrogels...
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SubjectTerms Bone and Bones
Cell Differentiation
Cells, Cultured
Chondroitin Sulfates - chemistry
Humans
Hydrogels
Mesenchymal Stem Cells
Osteogenesis
Tissue Engineering
Tissue Scaffolds
Title Chondroitin Sulfate-Based Biomineralizing Surface Hydrogels for Bone Tissue Engineering
URI http://dx.doi.org/10.1021/acsami.7b04114
https://www.ncbi.nlm.nih.gov/pubmed/28605908
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