DNA-Dependent Conformational Changes in the Ku Heterodimer

• Published in: Biochemistry (Easton) Vol. 47; no. 15; pp. 4359 - 4368
• Main Authors: Lehman, Jason A, Hoelz, Derek J, Turchi, John J
• Format: Journal Article
• Language: English
• Published: United States American Chemical Society 15.04.2008
• Subjects: Amino Acid Sequence; Antigens, Nuclear - chemistry; Biotinylation; Dimerization; DNA - chemistry; DNA-Binding Proteins - chemistry; Humans; Ku Autoantigen; Ligands; Lysine - chemistry; Mass Spectrometry; Models, Molecular; Molecular Sequence Data; Peptides - chemistry; Protein Binding; Protein Structure, Tertiary; Solvents - chemistry

Ionizing radiation induces DNA double-strand breaks which are repaired by the nonhomologous end joining (NHEJ) pathway. NHEJ is initiated upon Ku binding to the DNA ends and facilitating an interaction with the DNA-dependent protein kinase catalytic subunit (DNA-PKcs). This heterotrimeric DNA-PK com...