Immobilization of Enzymes through One-Pot Chemical Preoxidation and Electropolymerization of Dithiols in Enzyme-Containing Aqueous Suspensions To Develop Biosensors with Improved Performance

• Published in: Analytical chemistry (Washington) Vol. 80; no. 15; pp. 5829 - 5838
• Main Authors: Fu, Yingchun, Chen, Chao, Xie, Qingji, Xu, Xiahong, Zou, Can, Zhou, Qingmei, Tan, Liang, Tang, Hao, Zhang, Youyu, Yao, Shouzhuo
• Format: Journal Article
• Language: English
• Published: Washington, DC American Chemical Society 01.08.2008
• Subjects: Alkaline Phosphatase; Analytical chemistry; Aqueous solutions; Biofuels; Biological and medical sciences; Biomaterials; Biosensing Techniques - methods; Biosensors; Biotechnology; Chemistry; Electrochemical methods; Electrochemistry; Electrochemistry - methods; Electrodes; Enzymes; Enzymes, Immobilized - chemical synthesis; Enzymes, Immobilized - chemistry; Exact sciences and technology; Fundamental and applied biological sciences. Psychology; Glucose; Glucose - analysis; Glucose Oxidase; Methods. Procedures. Technologies; Organophosphates - analysis; Oxidation; Polymers - chemical synthesis; Spectrometric and optical methods; Suspensions; Toluene - analogs & derivatives; Toluene - chemistry; Various methods and equipments; Performance evaluation; Phosphates; Alkaline phosphatase; Glucose oxidase; Electrochemical method; In situ; Phosphoric monoester hydrolases; Immobilization; Esterases; Benzoquinone; Biofuel; Oligomer; Electrochemical polymerization; Aminophenols; Specific activity; Enzymatic activity; Amperometry; Enzyme; Enzyme electrode; Chromatography; Sensitivity; Biosensor; Hydrolases; Oxidoreductases; Aqueous solution; Spectrophotometry; Immobilized enzyme; Quartz microbalance; Ultraviolet visible spectrometry

A protocol of one-pot chemical preoxidation and electropolymerization of monomers (CPEM) in enzyme-containing aqueous suspensions (or solutions) was proposed as a universal strategy for high-activity and high-load immobilization of enzymes to construct amperometric biosensors, which was proven to be...