Uptake and Effects of Microplastics on Cells and Tissue of the Blue Mussel Mytilus edulis L. after an Experimental Exposure

• Published in: Environmental science & technology Vol. 46; no. 20; pp. 11327 - 11335
• Main Authors: von Moos, Nadia, Burkhardt-Holm, Patricia, Köhler, Angela
• Format: Journal Article
• Language: English
• Published: Washington, DC American Chemical Society 16.10.2012
• Subjects: Animal, plant and microbial ecology; Animals; Applied ecology; Biological and medical sciences; Biomarkers; Biomarkers - metabolism; Cells; Ecotoxicology, biological effects of pollution; Effects of pollution and side effects of pesticides on protozoa and invertebrates; Environmental Exposure - adverse effects; Fundamental and applied biological sciences. Psychology; Gills - metabolism; High density polyethylenes; Membranes; Microscopy; Mollusks; Mytilus edulis - metabolism; Plastic pollution; Plastics - metabolism; Plastics - toxicity; Polyethylene; Polyethylene - metabolism; Polyethylene - toxicity; Tissues; Water Pollutants, Chemical - metabolism; Water Pollutants, Chemical - toxicity; Mytilus edulis; Toxicity; Experimental study; Marine environment; Pollutant; Tissue; Absorption; Bivalvia; Microparticle; Invertebrata; Mollusca; Plastics; Cell
• DOI: 10.1021/es302332w
• ISSN: 0013-936X

In this study, we investigated if industrial high-density polyethylene (HDPE) particles, a model microplastic free of additives, ranging > 0–80 μm are ingested and taken up into the cells and tissue of the blue mussel Mytilus edulis L. The effects of exposure (up to 96 h) and plastic ingestion were observed at the cellular and subcellular level. Microplastic uptake into the gills and digestive gland was analyzed by a new method using polarized light microscopy. Mussel health status was investigated incorporating histological assessment and cytochemical biomarkers of toxic effects and early warning. In addition to being drawn into the gills, HDPE particles were taken up into the stomach and transported into the digestive gland where they accumulated in the lysosomal system after 3 h of exposure. Our results show notable histological changes upon uptake and a strong inflammatory response demonstrated by the formation of granulocytomas after 6 h and lysosomal membrane destabilization, which significantly increased with longer exposure times. We provide proof of principle that microplastics are taken up into cells and cause significant effects on the tissue and cellular level, which can be assessed with standard cytochemical biomarkers and polarized light microscopy for microplastic tracking in tissue.