In Situ Molecular Analysis of Plant Tissues by Live Single-Cell Mass Spectrometry

• Published in: Analytical chemistry (Washington) Vol. 84; no. 12; pp. 5221 - 5228
• Main Authors: Lorenzo Tejedor, Mónica, Mizuno, Hajime, Tsuyama, Naohiro, Harada, Takanori, Masujima, Tsutomu
• Format: Journal Article
• Language: English
• Published: Washington, DC American Chemical Society 19.06.2012
• Subjects: Analytical chemistry; Applied sciences; Cell Survival; Cells; Chemistry; Exact sciences and technology; Flowers & plants; Global environmental pollution; Mass spectrometry; Mass Spectrometry - methods; Metabolites; Molecules; Nanotechnology; Pelargonium - cytology; Pelargonium - metabolism; Plant Leaves - cytology; Plant Leaves - metabolism; Pollution; Single-Cell Analysis - methods; Spectrometric and optical methods; Microscope; Plant tissue; High resolution; Vegetals; Metabolite; Sample; In situ; Use; Plant leaf; Electrospray; Direct method; Molecules; Plant; Sample preparation; Ionization; Morphology; Environment; Peak; Mass spectrometry; Tool

We report the development of a rapid, direct molecular analysis of live, single plant cells viewed under a video microscope in their natural environment. A nanoelectrospray tip was used to extract the contents of a single leaf, stem, or petal cell from Pelargonium zonale, and the samples were analyz...