Label-Free Colorimetric Assay for Methyltransferase Activity Based on a Novel Methylation-Responsive DNAzyme Strategy

• Published in: Analytical chemistry (Washington) Vol. 82; no. 5; pp. 1935 - 1941
• Main Authors: Li, Wang, Liu, Zhuoliang, Lin, Hui, Nie, Zhou, Chen, Jinhua, Xu, Xiahong, Yao, Shouzhuo
• Format: Journal Article
• Language: English
• Published: Washington, DC American Chemical Society 01.03.2010
• Subjects: Analytical chemistry; Base Sequence; Chemistry; Colorimetry - methods; Deoxyribonucleic acid; DNA; DNA Methylation; DNA Modification Methylases - metabolism; DNA, Catalytic - metabolism; Electrophoresis, Polyacrylamide Gel; Enzyme kinetics; Exact sciences and technology; Spectrometric and optical methods; Studies; Tissue engineering; Catalytic reaction; Chemical analysis; Nucleotide sequence; Enzyme; Color; Activation; Colorimetry; Method; Gene expression; Probe; Gene amplification; Signal; Peroxidases; DNA; Detection limit; Restriction endonuclease; Strategy; Peroxidase; Oxidoreductases; Coupling; Inhibition; Detection; Methylation; Methyl transferases

DNA methylation catalyzed by methyltransferase (MTase) is a significant epigenetic process for modulating gene expression. Traditional methods to study MTase activity require a laborious and costly DNA labeling process. In this article, we report a simple, colorimetric, and label-free methylation-re...