Controlled Release of Drugs from Gradient Hydrogels for High-Throughput Analysis of Cell–Drug Interactions

• Published in: Analytical chemistry (Washington) Vol. 84; no. 3; pp. 1302 - 1309
• Main Authors: Ostrovidov, Serge, Annabi, Nasim, Seidi, Azadeh, Ramalingam, Murugan, Dehghani, Fariba, Kaji, Hirokazu, Khademhosseini, Ali
• Format: Journal Article
• Language: English
• Published: Washington, DC American Chemical Society 07.02.2012
• Subjects: Analytical chemistry; Animals; Cell Line; Cell Survival; Cells; Chemical reactions; Chemistry; Drugs; Exact sciences and technology; High-Throughput Screening Assays; Hydrogels - chemistry; Mice; Microfluidic Analytical Techniques; Okadaic Acid - chemistry; Polyethylene glycol; Polyethylene Glycols - chemistry; Studies; Drug; Fluid mechanics; Ethylene glycol; Use; Glass; Method; Slide; Concentration effect; Chemical concentration; Diagnosis; Hydrogel; Application; Viability; Release; Microfluidics; Concentration gradient

In this paper, we report a method to fabricate microengineered hydrogels that contain a concentration gradient of a drug for high-throughput analysis of cell-drug interactions. A microfluidic gradient generator was used to create a concentration gradient of okadaic acid (OA) as a model drug within p...