Controlled Release of Drugs from Gradient Hydrogels for High-Throughput Analysis of Cell–Drug Interactions

In this paper, we report a method to fabricate microengineered hydrogels that contain a concentration gradient of a drug for high-throughput analysis of cell-drug interactions. A microfluidic gradient generator was used to create a concentration gradient of okadaic acid (OA) as a model drug within p...

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Published inAnalytical chemistry (Washington) Vol. 84; no. 3; pp. 1302 - 1309
Main Authors Ostrovidov, Serge, Annabi, Nasim, Seidi, Azadeh, Ramalingam, Murugan, Dehghani, Fariba, Kaji, Hirokazu, Khademhosseini, Ali
Format Journal Article
LanguageEnglish
Published Washington, DC American Chemical Society 07.02.2012
Subjects
Analytical chemistry
Animals
Cell Line
Cell Survival
Cells
Chemical reactions
Chemistry
Drugs
Exact sciences and technology
High-Throughput Screening Assays
Hydrogels - chemistry
Mice
Microfluidic Analytical Techniques
Okadaic Acid - chemistry
Polyethylene glycol
Polyethylene Glycols - chemistry
Studies
Drug
Fluid mechanics
Ethylene glycol
Use
Glass
Method
Slide
Concentration effect
Chemical concentration
Diagnosis
Hydrogel
Application
Viability
Release
Microfluidics
Concentration gradient
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Abstract In this paper, we report a method to fabricate microengineered hydrogels that contain a concentration gradient of a drug for high-throughput analysis of cell-drug interactions. A microfluidic gradient generator was used to create a concentration gradient of okadaic acid (OA) as a model drug within poly(ethylene glycol) diacrylate hydrogels. These hydrogels were then incubated with MC3T3-E1 cell seeded glass slides to investigate the cell viability through the spatially controlled release of OA. The drug was released from the hydrogel in a gradient manner and induced a gradient of the cell viability. The drug concentration gradient containing hydrogels developed in this study have the potential to be used for drug discovery and diagnostics applications due to their ability to simultaneously test the effects of different concentrations of various chemicals.
AbstractList In this paper, we report a method to fabricate microengineered hydrogels that contain a concentration gradient of a drug for high-throughput analysis of cell-drug interactions. A microfluidic gradient generator was used to create a concentration gradient of okadaic acid (OA) as a model drug within poly(ethylene glycol) diacrylate hydrogels. These hydrogels were then incubated with MC3T3-E1 cell seeded glass slides to investigate the cell viability through the spatially controlled release of OA. The drug was released from the hydrogel in a gradient manner and induced a gradient of the cell viability. The drug concentration gradient containing hydrogels developed in this study have the potential to be used for drug discovery and diagnostics applications due to their ability to simultaneously test the effects of different concentrations of various chemicals.
In this paper, we report a method to fabricate microengineered hydrogels that contain a concentration gradient of a drug for high-throughput analysis of cell-drug interactions. A microfluidic gradient generator was used to create a concentration gradient of okadaic acid (OA) as a model drug within poly(ethylene glycol) diacrylate hydrogels. These hydrogels were then incubated with MC3T3-E1 cell seeded glass slides to investigate the cell viability through the spatially controlled release of OA. The drug was released from the hydrogel in a gradient manner and induced a gradient of the cell viability. The drug concentration gradient containing hydrogels developed in this study have the potential to be used for drug discovery and diagnostics applications due to their ability to simultaneously test the effects of different concentrations of various chemicals. [PUBLICATION ABSTRACT]
Author Seidi, Azadeh
Dehghani, Fariba
Ostrovidov, Serge
Kaji, Hirokazu
Ramalingam, Murugan
Khademhosseini, Ali
Annabi, Nasim
AuthorAffiliation Massachusetts Institute of Technology
University of Sydney
Harvard University
Tohoku University
Universite de Strasbourg
Harvard Medical School
AuthorAffiliation_xml – name: Harvard University
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  surname: Ostrovidov
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  givenname: Nasim
  surname: Annabi
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Issue 3
Keywords Drug
Fluid mechanics
Ethylene glycol
Use
Glass
Method
Slide
Concentration effect
Chemical concentration
Diagnosis
Hydrogel
Application
Viability
Release
Microfluidics
Concentration gradient
Language English
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2011 American Chemical Society
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Snippet In this paper, we report a method to fabricate microengineered hydrogels that contain a concentration gradient of a drug for high-throughput analysis of...
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SubjectTerms Analytical chemistry
Animals
Cell Line
Cell Survival
Cells
Chemical reactions
Chemistry
Drugs
Exact sciences and technology
High-Throughput Screening Assays
Hydrogels - chemistry
Mice
Microfluidic Analytical Techniques
Okadaic Acid - chemistry
Polyethylene glycol
Polyethylene Glycols - chemistry
Studies
Title Controlled Release of Drugs from Gradient Hydrogels for High-Throughput Analysis of Cell–Drug Interactions
URI http://dx.doi.org/10.1021/ac202256c
https://www.ncbi.nlm.nih.gov/pubmed/22220576
https://www.proquest.com/docview/922829502
https://search.proquest.com/docview/921146889
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