Controlled Release of Drugs from Gradient Hydrogels for High-Throughput Analysis of Cell–Drug Interactions

• Published in: Analytical chemistry (Washington) Vol. 84; no. 3; pp. 1302 - 1309
• Main Authors: Ostrovidov, Serge, Annabi, Nasim, Seidi, Azadeh, Ramalingam, Murugan, Dehghani, Fariba, Kaji, Hirokazu, Khademhosseini, Ali
• Format: Journal Article
• Language: English
• Published: Washington, DC American Chemical Society 07.02.2012
• Subjects: Analytical chemistry; Animals; Cell Line; Cell Survival; Cells; Chemical reactions; Chemistry; Drugs; Exact sciences and technology; High-Throughput Screening Assays; Hydrogels - chemistry; Mice; Microfluidic Analytical Techniques; Okadaic Acid - chemistry; Polyethylene glycol; Polyethylene Glycols - chemistry; Studies; Drug; Fluid mechanics; Ethylene glycol; Use; Glass; Method; Slide; Concentration effect; Chemical concentration; Diagnosis; Hydrogel; Application; Viability; Release; Microfluidics; Concentration gradient
• ISSN: 0003-2700; 1520-6882
• DOI: 10.1021/ac202256c

In this paper, we report a method to fabricate microengineered hydrogels that contain a concentration gradient of a drug for high-throughput analysis of cell-drug interactions. A microfluidic gradient generator was used to create a concentration gradient of okadaic acid (OA) as a model drug within poly(ethylene glycol) diacrylate hydrogels. These hydrogels were then incubated with MC3T3-E1 cell seeded glass slides to investigate the cell viability through the spatially controlled release of OA. The drug was released from the hydrogel in a gradient manner and induced a gradient of the cell viability. The drug concentration gradient containing hydrogels developed in this study have the potential to be used for drug discovery and diagnostics applications due to their ability to simultaneously test the effects of different concentrations of various chemicals.