Genomics-Guided Exploitation of Lipopeptide Diversity in Myxobacteria

Analysis of 122 myxobacterial genome sequences suggested 16 strains as producers of the myxochromide lipopeptide family. Detailed sequence comparison of the respective mch biosynthetic gene clusters informed a genome-mining approach, ultimately leading to the discovery and chemical characterization...

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Published inACS Chemical Biology Vol. 12; no. 3; pp. 779 - 786
Main Authors Burgard, Christian, Zaburannyi, Nestor, Nadmid, Suvd, Maier, Josef, Jenke-Kodama, Holger, Luxenburger, Eva, Bernauer, Hubert S, Wenzel, Silke C
Format Journal Article
LanguageEnglish
Published United States American Chemical Society 17.03.2017
American Chemical Society (ACS)
Subjects
Genomics
Lipopeptides
Lipopeptides - metabolism
Multigene Family
Myxococcales
Myxococcales - genetics
Myxococcales - metabolism
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Abstract Analysis of 122 myxobacterial genome sequences suggested 16 strains as producers of the myxochromide lipopeptide family. Detailed sequence comparison of the respective mch biosynthetic gene clusters informed a genome-mining approach, ultimately leading to the discovery and chemical characterization of four novel myxochromide core types. The myxochromide megasynthetase is subject to evolutionary diversification, resulting in considerable structural diversity of biosynthesis products. The observed differences are due to the number, type, sequence, and configuration of the incorporated amino acids. The analysis revealed molecular details on how point mutations and recombination events led to structural diversity. It also gave insights into the evolutionary scenarios that have led to the emergence of mch clusters in different strains and genera of myxobacteria.
AbstractList Analysis of 122 myxobacterial genome sequences suggested 16 strains as producers of the myxochromide lipopeptide family. Detailed sequence comparison of the respective mch biosynthetic gene clusters informed a genome-mining approach, ultimately leading to the discovery and chemical characterization of four novel myxochromide core types. The myxochromide megasynthetase is subject to evolutionary diversification, resulting in considerable structural diversity of biosynthesis products. The observed differences are due to the number, type, sequence, and configuration of the incorporated amino acids. The analysis revealed molecular details on how point mutations and recombination events led to structural diversity. It also gave insights into the evolutionary scenarios that have led to the emergence of mch clusters in different strains and genera of myxobacteria.Analysis of 122 myxobacterial genome sequences suggested 16 strains as producers of the myxochromide lipopeptide family. Detailed sequence comparison of the respective mch biosynthetic gene clusters informed a genome-mining approach, ultimately leading to the discovery and chemical characterization of four novel myxochromide core types. The myxochromide megasynthetase is subject to evolutionary diversification, resulting in considerable structural diversity of biosynthesis products. The observed differences are due to the number, type, sequence, and configuration of the incorporated amino acids. The analysis revealed molecular details on how point mutations and recombination events led to structural diversity. It also gave insights into the evolutionary scenarios that have led to the emergence of mch clusters in different strains and genera of myxobacteria.
Analysis of 122 myxobacterial genome sequences suggested 16 strains as producers of the myxochromide lipopeptide family. Detailed sequence comparison of the respective mch biosynthetic gene clusters informed a genome-mining approach, ultimately leading to the discovery and chemical characterization of four novel myxochromide core types. The myxochromide megasynthetase is subject to evolutionary diversification, resulting in considerable structural diversity of biosynthesis products. The observed differences are due to the number, type, sequence, and configuration of the incorporated amino acids. The analysis revealed molecular details on how point mutations and recombination events led to structural diversity. It also gave insights into the evolutionary scenarios that have led to the emergence of mch clusters in different strains and genera of myxobacteria.
Author Zaburannyi, Nestor
Wenzel, Silke C
Luxenburger, Eva
Maier, Josef
Jenke-Kodama, Holger
Bernauer, Hubert S
Burgard, Christian
Nadmid, Suvd
AuthorAffiliation Okinawa Institute of Science and Technology (OIST)
IStLS − Information Services to Life Sciences
Helmholtz Institute for Pharmaceutical Research Saarland (HIPS), Helmholtz Centre for Infection Research and Pharmaceutical Biotechnology at Saarland University
ATG Biosynthetics GmbH
Microbiology and Biochemistry of Secondary Metabolites Unit
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Snippet Analysis of 122 myxobacterial genome sequences suggested 16 strains as producers of the myxochromide lipopeptide family. Detailed sequence comparison of the...
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SubjectTerms Genomics
Lipopeptides
Lipopeptides - metabolism
Multigene Family
Myxococcales
Myxococcales - genetics
Myxococcales - metabolism
Title Genomics-Guided Exploitation of Lipopeptide Diversity in Myxobacteria
URI http://dx.doi.org/10.1021/acschembio.6b00953
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