Rapid and High-Sensitivity Cell-Based Assays of Protein−Protein Interactions Using Split Click Beetle Luciferase Complementation: An Approach to the Study of G-Protein-Coupled Receptors

• Published in: Analytical chemistry (Washington) Vol. 82; no. 6; pp. 2552 - 2560
• Main Authors: Misawa, Naomi, Kafi, A. K. M, Hattori, Mitsuru, Miura, Kenji, Masuda, Kenji, Ozawa, Takeaki
• Format: Journal Article
• Language: English
• Published: Washington, DC American Chemical Society 15.03.2010
• Subjects: Analytical chemistry; Animals; Arrestins - genetics; Arrestins - metabolism; beta-Arrestins; Bioluminescence; Cell Line; Cells; Chemical compounds; Chemistry; Coleoptera - enzymology; Enzymes; Exact sciences and technology; Gene Expression; Humans; Insects; Luciferases - chemistry; Luciferases - metabolism; Membranes; Protein Interaction Mapping - methods; Proteins; Receptors, G-Protein-Coupled - genetics; Receptors, G-Protein-Coupled - metabolism; Sensitivity and Specificity; Spatial analysis; Fold; Bioluminescence; Chemical analysis; Microtiter plate; Ligand; Molecular interaction; Stimulation; Biology; Time; Signal; Monitoring; G protein; Drug; Enzyme; Luciferase; Method; Screening; Sensitivity; Cell line; Microscopy; Animal; Plasma membrane; Oxidoreductases; Library; Intracellular; Technique

To identify biologically relevant compounds in basic biology and drug discovery processes, rapid quantitative methods for elucidating protein−protein interactions have become necessary. We describe a novel optical technique for monitoring protein−protein interactions in living cells based on complem...