Detection and Quantification of Ricin in Beverages Using Isotope Dilution Tandem Mass Spectrometry
The toxic plant protein ricin has gained notoriety due to wide availability and potential use as a bioterrorism agent, with particular concern for food supply contamination. We have developed a sensitive and selective mass spectrometry-based method to detect ricin in tap water, 2% milk, apple juice,...
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Published in | Analytical chemistry (Washington) Vol. 83; no. 8; pp. 2897 - 2905 |
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Main Authors | , , , , |
Format | Journal Article |
Language | English |
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Washington, DC
American Chemical Society
15.04.2011
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Abstract | The toxic plant protein ricin has gained notoriety due to wide availability and potential use as a bioterrorism agent, with particular concern for food supply contamination. We have developed a sensitive and selective mass spectrometry-based method to detect ricin in tap water, 2% milk, apple juice, and orange juice. Ricin added to beverage matrices was extracted using antibody-bound magnetic beads and digested with trypsin. Absolute quantification was performed using isotope dilution mass spectrometry with a linear ion trap operating in product-ion-monitoring mode. The method allows for identification of ricin A chain and B chain and for distinction of ricin from ricin agglutinin within a single analytical run. Ricin-bound beads were also tested for deadenylase activity by incubation with a synthetic ssDNA oligomer. Depurination of the substrate by ricin was confirmed by matrix-assisted laser desorption ionization-time-of-flight mass spectrometry (MALDI-TOFMS). This method was used successfully to extract ricin from each beverage matrix. The activity of recovered ricin was assessed, and quantification was achieved, with a limit of detection of 10 fmol/mL (0.64 ng/mL). |
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AbstractList | The toxic plant protein ricin has gained notoriety due to wide availability and potential use as a bioterrorism agent, with particular concern for food supply contamination. We have developed a sensitive and selective mass spectrometry-based method to detect ricin in tap water, 2% milk, apple juice, and orange juice. Ricin added to beverage matrices was extracted using antibody-bound magnetic beads and digested with trypsin. Absolute quantification was performed using isotope dilution mass spectrometry with a linear ion trap operating in product-ion-monitoring mode. The method allows for identification of ricin A chain and B chain and for distinction of ricin from ricin agglutinin within a single analytical run. Ricin-bound beads were also tested for deadenylase activity by incubation with a synthetic ssDNA oligomer. Depurination of the substrate by ricin was confirmed by matrix-assisted laser desorption ionization-time-of-flight mass spectrometry (MALDI-TOFMS). This method was used successfully to extract ricin from each beverage matrix. The activity of recovered ricin was assessed, and quantification was achieved, with a limit of detection of 10 fmol/mL (0.64 ng/mL). The toxic plant protein ricin has gained notoriety due to wide availability and potential use as a bioterrorism agent, with particular concern for food supply contamination. We have developed a sensitive and selective mass spectrometry-based method to detect ricin in tap water, 2% milk, apple juice, and orange juice. Ricin added to beverage matrices was extracted using antibody-bound magnetic beads and digested with trypsin. Absolute quantification was performed using isotope dilution mass spectrometry with a linear ion trap operating in product-ion-monitoring mode. The method allows for identification of ricin A chain and B chain and for distinction of ricin from ricin agglutinin within a single analytical run. Ricin-bound beads were also tested for deadenylase activity by incubation with a synthetic ssDNA oligomer. Depurination of the substrate by ricin was confirmed by matrix-assisted laser desorption ionization-time-of-flight mass spectrometry (MALDI-TOFMS). This method was used successfully to extract ricin from each beverage matrix. The activity of recovered ricin was assessed, and quantification was achieved, with a limit of detection of 10 fmol/mL (0.64 ng/mL). [PUBLICATION ABSTRACT] |
Author | Barr, John R McGrath, Sara C Pirkle, James L Schieltz, David M McWilliams, Lisa G |
Author_xml | – sequence: 1 givenname: Sara C surname: McGrath fullname: McGrath, Sara C – sequence: 2 givenname: David M surname: Schieltz fullname: Schieltz, David M – sequence: 3 givenname: Lisa G surname: McWilliams fullname: McWilliams, Lisa G – sequence: 4 givenname: James L surname: Pirkle fullname: Pirkle, James L – sequence: 5 givenname: John R surname: Barr fullname: Barr, John R email: jbarr@cdc.gov |
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Keywords | Identification Matrix assisted laser desorption ionization Oligomer Ion trap Plant Sample preparation Castor (seed) Detection limit Beverage Monitoring Quantitative analysis Milk Vegetals Antibody Isotope dilution Foodstuff Tap water Contamination Protein Substrate Apple Time of flight method Mass spectrometry MS/MS Simultaneous measurement Mass spectrometry |
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Snippet | The toxic plant protein ricin has gained notoriety due to wide availability and potential use as a bioterrorism agent, with particular concern for food supply... |
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SubjectTerms | Analytical chemistry Beverages Beverages - analysis Chemistry Exact sciences and technology Food contamination & poisoning Humans Isotope Labeling - methods Isotopes Mass spectrometry Proteins Ricin - analysis Spectrometric and optical methods Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization |
Title | Detection and Quantification of Ricin in Beverages Using Isotope Dilution Tandem Mass Spectrometry |
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