A New Methodology for Quantitative LSPR Biosensing and Imaging

• Published in: Analytical chemistry (Washington) Vol. 84; no. 3; pp. 1367 - 1373
• Main Authors: Raphael, Marc P., Christodoulides, Joseph A., Mulvaney, Shawn P., Miller, Michael M., Long, James P., Byers, Jeff M.
• Format: Journal Article
• Language: English
• Published: Washington, DC American Chemical Society 07.02.2012
• Subjects: Analytical chemistry; Avidin - analysis; Avidin - metabolism; Binding sites; Biological and medical sciences; Biosensing Techniques - methods; Biosensors; Biotechnology; Biotin - metabolism; Chemistry; Electrons; Exact sciences and technology; Fundamental and applied biological sciences. Psychology; General, instrumentation; Gold - chemistry; Kinetics; Metal Nanoparticles - chemistry; Methods. Procedures. Technologies; Scattering; Spectrum analysis; Surface Plasmon Resonance - methods; Surface Properties; Various methods and equipments; Disposable; Gold; Sample; Use; Glass; Electron beam; Nanostructure; Time; Concentration; Chemical sensor; Array; Biosensor; Imaging; Coverslip; Peak; Surface plasmon resonance; Kinetics; Technique; Spatial resolution; Comparative study; Quantitative analysis

A new quantitative analysis methodology for localized surface plasmon resonance (LSPR) biosensing which determines surface-receptor fractional occupancy, as well as an LSPR imaging technique for the spatiotemporal mapping of binding events, is presented. Electron beam nanolithography was used to fab...