Label-Free, Electrical Detection of the SARS Virus N-Protein with Nanowire Biosensors Utilizing Antibody Mimics as Capture Probes

• Published in: ACS nano Vol. 3; no. 5; pp. 1219 - 1224
• Main Authors: Ishikawa, Fumiaki N, Chang, Hsiao-Kang, Curreli, Marco, Liao, Hsiang-I, Olson, C. Anders, Chen, Po-Chiang, Zhang, Rui, Roberts, Richard W, Sun, Ren, Cote, Richard J, Thompson, Mark E, Zhou, Chongwu
• Format: Journal Article
• Language: English
• Published: United States American Chemical Society 26.05.2009
• Subjects: Antibodies, Viral - chemistry; Biomimetic Materials - chemistry; Biosensing Techniques - instrumentation; Electrochemistry - instrumentation; Equipment Design; Fibronectins - immunology; Indium - chemistry; Macromolecular Substances - chemistry; Molecular Conformation; Nanostructures - chemistry; Nanostructures - ultrastructure; Nanotechnology - instrumentation; Particle Size; SARS coronavirus; Severe acute respiratory syndrome-related coronavirus - immunology; Severe acute respiratory syndrome-related coronavirus - isolation & purification; Staining and Labeling; Surface Properties; antibody mimetic protein; biosensor; nucleocapsid (N) protein; nanowire

Antibody mimic proteins (AMPs) are polypeptides that bind to their target analytes with high affinity and specificity, just like conventional antibodies, but are much smaller in size (2−5 nm, less than 10 kDa). In this report, we describe the first application of AMP in the field of nanobiosensors....