Label-Free, Electrical Detection of the SARS Virus N-Protein with Nanowire Biosensors Utilizing Antibody Mimics as Capture Probes
Antibody mimic proteins (AMPs) are polypeptides that bind to their target analytes with high affinity and specificity, just like conventional antibodies, but are much smaller in size (2−5 nm, less than 10 kDa). In this report, we describe the first application of AMP in the field of nanobiosensors....
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| Published in | ACS nano Vol. 3; no. 5; pp. 1219 - 1224 |
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| Main Authors | , , , , , , , , , , , |
| Format | Journal Article |
| Language | English |
| Published |
United States
American Chemical Society
26.05.2009
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| Subjects | |
| Online Access | Get full text |
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| Abstract | Antibody mimic proteins (AMPs) are polypeptides that bind to their target analytes with high affinity and specificity, just like conventional antibodies, but are much smaller in size (2−5 nm, less than 10 kDa). In this report, we describe the first application of AMP in the field of nanobiosensors. In2O3 nanowire based biosensors have been configured with an AMP (Fibronectin, Fn) to detect nucleocapsid (N) protein, a biomarker for severe acute respiratory syndrome (SARS). Using these devices, N protein was detected at subnanomolar concentration in the presence of 44 μM bovine serum albumin as a background. Furthermore, the binding constant of the AMP to Fn was determined from the concentration dependence of the response of our biosensors. |
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| AbstractList | Antibody mimic proteins (AMPs) are polypeptides that bind to their target analytes with high affinity and specificity, just like conventional antibodies, but are much smaller in size (2-5 nm, less than 10 kDa). In this report, we describe the first application of AMP in the field of nanobiosensors. In(2)O(3) nanowire based biosensors have been configured with an AMP (Fibronectin, Fn) to detect nucleocapsid (N) protein, a biomarker for severe acute respiratory syndrome (SARS). Using these devices, N protein was detected at subnanomolar concentration in the presence of 44 microM bovine serum albumin as a background. Furthermore, the binding constant of the AMP to Fn was determined from the concentration dependence of the response of our biosensors. Antibody mimic proteins (AMPs) are poly-peptides that bind to their target analytes with high affinity and specificity, just like conventional antibodies, but are much smaller in size (2–5 nm, less than 10kDa). In this report, we describe the first application of AMP in the field of nanobiosensors. In 2 O 3 nanowire based biosensors have been configured with an AMP (Fibronectin, Fn) to detect nucleocapsid (N) protein, a biomarker for severe acute respiratory syndrome (SARS). Using these devices, N protein was detected at sub-nanomolar concentration in the presence of 44 µM bovine serum albumin as a background. Furthermore, the binding constant of the AMP to Fn was determined from the concentration dependence of the response of our biosensors. Antibody mimic proteins (AMPs) are polypeptides that bind to their target analytes with high affinity and specificity, just like conventional antibodies, but are much smaller in size (2-5 nm, less than 10 kDa). In this report, we describe the first application of AMP in the field of nanobiosensors. In2O3 nanowire based biosensors have been configured with an AMP (Fibronectin, Fn) to detect nucleocapsid (N) protein, a biomarker for severe acute respiratory syndrome (SARS). Using these devices, N protein was detected at subnanomolar concentration in the presence of 44 *mM bovine serum albumin as a background. Furthermore, the binding constant of the AMP to Fn was determined from the concentration dependence of the response of our biosensors. Antibody mimic proteins (AMPs) are polypeptides that bind to their target analytes with high affinity and specificity, just like conventional antibodies, but are much smaller in size (2-5 nm, less than 10 kDa). In this report, we describe the first application of AMP in the field of nanobiosensors. In(2)O(3) nanowire based biosensors have been configured with an AMP (Fibronectin, Fn) to detect nucleocapsid (N) protein, a biomarker for severe acute respiratory syndrome (SARS). Using these devices, N protein was detected at subnanomolar concentration in the presence of 44 microM bovine serum albumin as a background. Furthermore, the binding constant of the AMP to Fn was determined from the concentration dependence of the response of our biosensors.Antibody mimic proteins (AMPs) are polypeptides that bind to their target analytes with high affinity and specificity, just like conventional antibodies, but are much smaller in size (2-5 nm, less than 10 kDa). In this report, we describe the first application of AMP in the field of nanobiosensors. In(2)O(3) nanowire based biosensors have been configured with an AMP (Fibronectin, Fn) to detect nucleocapsid (N) protein, a biomarker for severe acute respiratory syndrome (SARS). Using these devices, N protein was detected at subnanomolar concentration in the presence of 44 microM bovine serum albumin as a background. Furthermore, the binding constant of the AMP to Fn was determined from the concentration dependence of the response of our biosensors. Antibody mimic proteins (AMPs) are polypeptides that bind to their target analytes with high affinity and specificity, just like conventional antibodies, but are much smaller in size (2−5 nm, less than 10 kDa). In this report, we describe the first application of AMP in the field of nanobiosensors. In2O3 nanowire based biosensors have been configured with an AMP (Fibronectin, Fn) to detect nucleocapsid (N) protein, a biomarker for severe acute respiratory syndrome (SARS). Using these devices, N protein was detected at subnanomolar concentration in the presence of 44 μM bovine serum albumin as a background. Furthermore, the binding constant of the AMP to Fn was determined from the concentration dependence of the response of our biosensors. |
| Author | Sun, Ren Liao, Hsiang-I Roberts, Richard W Chen, Po-Chiang Ishikawa, Fumiaki N Curreli, Marco Chang, Hsiao-Kang Zhang, Rui Olson, C. Anders Cote, Richard J Zhou, Chongwu Thompson, Mark E |
| AuthorAffiliation | c Department of Pathology, University of Southern California, Los Angeles, CA 90089 a Department of Electrical Engineering, University of Southern California, Los Angeles, CA 90089 d Department of Molecular and Medical Pharmacology, University of California Los Angeles, Los Angels, CA 90095 b Department of Chemistry, University of Southern California, Los Angeles, CA 90089 |
| AuthorAffiliation_xml | – name: a Department of Electrical Engineering, University of Southern California, Los Angeles, CA 90089 – name: b Department of Chemistry, University of Southern California, Los Angeles, CA 90089 – name: d Department of Molecular and Medical Pharmacology, University of California Los Angeles, Los Angels, CA 90095 – name: c Department of Pathology, University of Southern California, Los Angeles, CA 90089 |
| Author_xml | – sequence: 1 givenname: Fumiaki N surname: Ishikawa fullname: Ishikawa, Fumiaki N – sequence: 2 givenname: Hsiao-Kang surname: Chang fullname: Chang, Hsiao-Kang – sequence: 3 givenname: Marco surname: Curreli fullname: Curreli, Marco – sequence: 4 givenname: Hsiang-I surname: Liao fullname: Liao, Hsiang-I – sequence: 5 givenname: C. Anders surname: Olson fullname: Olson, C. Anders – sequence: 6 givenname: Po-Chiang surname: Chen fullname: Chen, Po-Chiang – sequence: 7 givenname: Rui surname: Zhang fullname: Zhang, Rui – sequence: 8 givenname: Richard W surname: Roberts fullname: Roberts, Richard W – sequence: 9 givenname: Ren surname: Sun fullname: Sun, Ren – sequence: 10 givenname: Richard J surname: Cote fullname: Cote, Richard J – sequence: 11 givenname: Mark E surname: Thompson fullname: Thompson, Mark E email: chongwuz@usc.edu, met@usc.edu – sequence: 12 givenname: Chongwu surname: Zhou fullname: Zhou, Chongwu email: chongwuz@usc.edu, met@usc.edu |
| BackLink | https://www.ncbi.nlm.nih.gov/pubmed/19422193$$D View this record in MEDLINE/PubMed |
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| Snippet | Antibody mimic proteins (AMPs) are polypeptides that bind to their target analytes with high affinity and specificity, just like conventional antibodies, but... Antibody mimic proteins (AMPs) are poly-peptides that bind to their target analytes with high affinity and specificity, just like conventional antibodies, but... |
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| SubjectTerms | Antibodies, Viral - chemistry Biomimetic Materials - chemistry Biosensing Techniques - instrumentation Electrochemistry - instrumentation Equipment Design Fibronectins - immunology Indium - chemistry Macromolecular Substances - chemistry Molecular Conformation Nanostructures - chemistry Nanostructures - ultrastructure Nanotechnology - instrumentation Particle Size SARS coronavirus Severe acute respiratory syndrome-related coronavirus - immunology Severe acute respiratory syndrome-related coronavirus - isolation & purification Staining and Labeling Surface Properties |
| Title | Label-Free, Electrical Detection of the SARS Virus N-Protein with Nanowire Biosensors Utilizing Antibody Mimics as Capture Probes |
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