Substrate Activation of Brewers' Yeast Pyruvate Decarboxylase Is Abolished by Mutation of Cysteine 221 to Serine - Baburina, Irina; Gao, Yuhong; Hu, Zhixiang; Jordan, Frank; Hohmann, Stefan; Furey, William (Biochemistry (Easton), 1994)
Keywords: ACTIVIDAD ENZIMATICA; ACTIVITE ENZYMATIQUE; Base Sequence; Binding Sites; CISTEINA; COENZIMAS; COENZYME; coenzymes; CYSTEINE; Cysteine - metabolism; DNA Primers; Enzyme Activation; enzyme activity; enzymology; Escherichia coli; genetics; Kinetics; LIASAS; LYASE; metabolism; Molecular Sequence Data; MUTACION; MUTACION INDUCIDA; MUTANT; MUTANTES; mutants; MUTATION; MUTATION PROVOQUEE; pyruvate decarboxylase; Pyruvate Decarboxylase - genetics; Pyruvate Decarboxylase - metabolism; Recombinant Proteins; Recombinant Proteins - metabolism; SACCHAROMYCES CEREVISIAE; Saccharomyces cerevisiae - enzymology; Saccharomyces cerevisiae - genetics; SERINA; SERINE; Serine - metabolism; site-directed mutagenesis; Substrate Specificity; THIAMINE; TIAMINA