Comparative postantibacterial activities of pefloxacin, ciprofloxacin, and ofloxacin against intracellular multiplication of Legionella pneumophila serogroup 1

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Published inAntimicrobial Agents and Chemotherapy Vol. 34; no. 9; pp. 1733 - 1738
Main Authors RAJAGOPALAN-LEVASSEUR, P, DOURNON, E, DAMERON, G, VILDE, POCIDALO, J.-J
Format Journal Article
LanguageEnglish
Published Washington, DC American Society for Microbiology 01.09.1990
Subjects
Antibacterial agents
Antibiotics. Antiinfectious agents. Antiparasitic agents
Biological and medical sciences
Cell Division - drug effects
Ciprofloxacin
Ciprofloxacin - pharmacology
Humans
Intracellular Fluid
Intracellular Fluid - physiology
Legionella
Legionella - classification
Legionella - drug effects
Legionella - physiology
Macrophages - physiology
Medical sciences
Microbial Sensitivity Tests
Ofloxacin
Ofloxacin - pharmacology
Pefloxacin
Pefloxacin - pharmacology
Pharmacology. Drug treatments
Research Article
Serotyping
Time Factors
Antibiotic
Microorganism growth
Sensitivity resistance
Legionellaceae
Minimum inhibitory concentration
Microorganism culture
Bacteria
Legionella pneumophila
Infected cell
Antibacterial agent
In vitro
Macrophage
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AbstractList The inhibitory and postantibacterial activities of pefloxacin, ciprofloxacin, and ofloxacin against virulent Legionella pneumophila serogroup 1 were evaluated in cell-free and cellular models. In the absence of macrophages (with the tissue culture medium alone), bacterial numbers remained unchanged at 24 h in the presence of 0.1 microgram of pefloxacin, ciprofloxacin, or ofloxacin per ml and 1.0 microgram of pefloxacin per ml, whereas they were reduced in the presence of 1.0 microgram of ciprofloxacin or ofloxacin per ml. Experiments to evaluate the postantibacterial effects of these drugs were therefore performed with concentrations of 0.1 microgram/ml. In the cell-free model, brief exposure (1 h) of bacteria to each antimicrobial agent resulted in a transient decrease in numbers followed by logarithmic growth. In the cellular model, all three drugs (at 0.1 and 1.0 microgram/ml) inhibited the intracellular multiplication of L. pneumophila. The intracellular postantibacterial effects of 0.1 microgram of pefloxacin, ciprofloxacin, and ofloxacin per ml, which were left in contact with L. pneumophila-infected human macrophages for 24 h, were evaluated at various times after removal of the drugs. Pefloxacin was found to exhibit a significant inhibitory effect at 72 h, whereas following the removal of ciprofloxacin and ofloxacin, rapid bacterial multiplication occurred, leading to the destruction of the macrophage monolayer within 48 h. Thus, while pefloxacin, ciprofloxacin, and ofloxacin all inhibited the multiplication of L. pneumophila in human monocyte-derived macrophages, only pefloxacin exhibited a prolonged postantibacterial effect.
The inhibitory and postantibacterial activities of pefloxacin, ciprofloxacin, and ofloxacin against virulent Legionella pneumophila serogroup 1 were evaluated in cell-free and cellular models. In the absence of macrophages, bacterial numbers remained unchanged at 24 h in the presence of 0.1 mu g of pefloxacin, ciprofloxacin, or ofloxacin per ml and 1.0 mu g of pefloxacin per ml, whereas they were reduced in the presence of 1.0 mu g of ciprofloxacin per ml. Experiments to evaluate the postantibacterial effects of these drugs were therefore performed with concentrations of 0.1 mu g /ml. In the cell-free model, brief exposure (1 hr) of bacteria to each antimicrobial agent resulted in a transient decrease in numbers followed by logarithmic growth. In the cellular model, all three drugs (at 0.1 and 1.0 mu g/ml) inhibited the intracellular multiplication of L. pneumophila . The intracellular postantibacterial effects of 0.1 mu g of pefloxacin, ciprofloxacin, and ofloxacin per ml, which were left in contact with L. pneumophila -infected human macrophages for 24 h, were evaluated at various times after removal of the drugs.
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Author J J Pocidalo
E Dournon
P Rajagopalan-Levasseur
G Dameron
J L Vilde
AuthorAffiliation Hôpital Claude Bernard, Université VII, Institut National de la Santé et de la Recherche Médicale, Unité 13, France
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Issue 9
Keywords Antibiotic
Microorganism growth
Sensitivity resistance
Legionellaceae
Minimum inhibitory concentration
Microorganism culture
Bacteria
Legionella pneumophila
Infected cell
Antibacterial agent
In vitro
Macrophage
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The inhibitory and postantibacterial activities of pefloxacin, ciprofloxacin, and ofloxacin against virulent Legionella pneumophila serogroup 1 were evaluated...
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SubjectTerms Antibacterial agents
Antibiotics. Antiinfectious agents. Antiparasitic agents
Biological and medical sciences
Cell Division - drug effects
Ciprofloxacin
Ciprofloxacin - pharmacology
Humans
Intracellular Fluid
Intracellular Fluid - physiology
Legionella
Legionella - classification
Legionella - drug effects
Legionella - physiology
Macrophages - physiology
Medical sciences
Microbial Sensitivity Tests
Ofloxacin
Ofloxacin - pharmacology
Pefloxacin
Pefloxacin - pharmacology
Pharmacology. Drug treatments
Research Article
Serotyping
Time Factors
Title Comparative postantibacterial activities of pefloxacin, ciprofloxacin, and ofloxacin against intracellular multiplication of Legionella pneumophila serogroup 1
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