Black-Tea Polyphenols Suppress Postprandial Hypertriacylglycerolemia by Suppressing Lymphatic Transport of Dietary Fat in Rats

• Published in: Journal of agricultural and food chemistry Vol. 57; no. 15; pp. 7131 - 7136
• Main Authors: Kobayashi, Makoto, Ichitani, Masaki, Suzuki, Yuko, Unno, Tomonori, Sugawara, Takashi, Yamahira, Takashi, Kato, Masaki, Takihara, Takanobu, Sagesaka, Yuko, Kakuda, Takami, Ikeda, Ikuo
• Format: Journal Article
• Language: English
• Published: Washington, DC American Chemical Society 12.08.2009
• Subjects: animal models; Animals; Biological and medical sciences; Biological Transport; black tea; blood lipids; Camellia sinensis - chemistry; Coffee, tea and other stimulative beverage industries; dietary fat; Dietary Fats - metabolism; enzyme inhibition; Flavonoids - administration & dosage; Flavonoids - chemistry; Flavonoids - metabolism; Food industries; Fundamental and applied biological sciences. Psychology; General aspects; Handling, storage, packaging, transport; Humans; hypertriacylglycerolemia; Hypertriglyceridemia - drug therapy; Hypertriglyceridemia - metabolism; Lymph - metabolism; lymphatic system; lymphatic transport; Male; Molecular Nutrition; nutrient transport; Phenols - administration & dosage; Phenols - chemistry; Phenols - metabolism; Polyphenols; Postprandial Period; postprandial state; Rats; Rats, Sprague-Dawley; Rats, Wistar; Tea - chemistry; Tea - metabolism; triacylglycerol lipase; Black-tea polyphenols; rats; postprandial hypertriacylglycerolemia; theaflavins; pancreatic lipase; Flavanol; Rat; Enzyme; Postprandial; Rodentia; Triacylglycerol lipase; Esterases; Black tea; Flavonoid; Carboxylic ester hydrolases; Vertebrata; Polyphenol; Mammalia; Theaflavin; Pigments; Fat; Hydrolases; Transport
• ISSN: 0021-8561; 1520-5118
• DOI: 10.1021/jf900855v

Administration of black-tea polyphenols (BTP) at 100 and 200 mg/kg of body weight in rats suppressed postprandial hypertriacylglycerolemia in a dose-dependent manner. Administration of BTP also suppressed lymphatic recovery of 14C-trioleoylglycerol in rats that were cannulated in the thoracic duct. BTP dose-dependently inhibited the activity of pancreatic lipase in vitro with an IC50 of 0.254 mg/mL. When purified theaflavins, which are components of BTP, were used, theaflavins with galloyl moieties, but not those without galloyl moiety, inhibited the activity of pancreatic lipase. Theaflavin-3,3′-digallate (TFDG) was more effective in inhibiting the activity of pancreatic lipase than epigallocatechin gallate (EGCG), epicatechin gallate (ECG), and a mixture of EGCG and ECG. BTP and TFDG had a similar effect in inhibiting the activity of pancreatic lipase when the total polyphenol amount was adjusted to the same. BTP had no effect on micellar solubility of hydrolysis products of triacylglycerol. These results suggest that BTP suppressed postprandial hypertriacylglycerolemia by reducing triacylglycerol absorption via the inhibition of pancreatic lipase activity.