Structure-Guided Functional Characterization of Enediyne Self-Sacrifice Resistance Proteins, CalU16 and CalU19

• Published in: ACS chemical biology Vol. 9; no. 10; pp. 2347 - 2358
• Main Authors: Elshahawi, Sherif I, Ramelot, Theresa A, Seetharaman, Jayaraman, Chen, Jing, Singh, Shanteri, Yang, Yunhuang, Pederson, Kari, Kharel, Madan K, Xiao, Rong, Lew, Scott, Yennamalli, Ragothaman M, Miller, Mitchell D, Wang, Fengbin, Tong, Liang, Montelione, Gaetano T, Kennedy, Michael A, Bingman, Craig A, Zhu, Haining, Phillips, George N, Thorson, Jon S
• Format: Journal Article
• Language: English
• Published: United States American Chemical Society 17.10.2014
• Subjects: Anti-Bacterial Agents - pharmacology; Bacterial Proteins - chemistry; Bacterial Proteins - genetics; Bacterial Proteins - metabolism; Crystallography, X-Ray; Drug Resistance, Bacterial - genetics; Enediynes - pharmacology; Genomics - methods; Lipids - chemistry; Micromonospora - growth & development; Micromonospora - metabolism; Models, Molecular; Molecular Structure; Multigene Family; Mutagenesis, Site-Directed; Mutation - genetics; Protein Structure, Tertiary; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization

Calicheamicin γ1 I (1) is an enediyne antitumor compound produced by Micromonospora echinospora spp. calichensis, and its biosynthetic gene cluster has been previously reported. Despite extensive analysis and biochemical study, several genes in the biosynthetic gene cluster of 1 remain functionally...