Adsorption of the Protein Bovine Serum Albumin in a Planar Poly(acrylic acid) Brush Layer As Measured by Optical Reflectometry

• Published in: Langmuir Vol. 24; no. 13; pp. 6575 - 6584
• Main Authors: de Vos, Wiebe M, Biesheuvel, P. Maarten, de Keizer, Arie, Kleijn, J. Mieke, Cohen Stuart, Martien A
• Format: Journal Article
• Language: English
• Published: Washington, DC American Chemical Society 01.07.2008
• Subjects: Acrylic Resins - chemistry; Adsorption; Animals; aqueous-solution; Cattle; charge regulation; Chemistry; Chemistry Techniques, Analytical; chloride; Colloidal state and disperse state; complexes; electrostatic interactions; Exact sciences and technology; General and physical chemistry; Hydrogen-Ion Concentration; Interfaces: Adsorption, Reactions, Films, Forces; Optics and Photonics; Osmolar Concentration; particles; Salts; Serum Albumin, Bovine - chemistry; spectroscopy; spherical polyelectrolyte brushes; Surface physical chemistry; Experimental data; Critical value; Bovine; Excluded volume; Acrylic acid; Chain length; Prediction; Polymer; Serum albumin; Density; Grafting; Protein; Polyelectrolyte; Vertebrata; Mammalia; Adsorption; Ionic strength; Distribution; Reflectometry; pH; Models; Artiodactyla; Electrostatic potential; Ungulata

The adsorption of bovine serum albumin (BSA) in a planar poly(acrylic acid) (PAA) brush layer has been studied by fixed-angle optical reflectometry. The influence of polymer length, grafting density, and salt concentration is studied as a function of pH. The results are compared with predictions of...