Comprehensive Identification of Glycated Peptides and Their Glycation Motifs in Plasma and Erythrocytes of Control and Diabetic Subjects

• Published in: Journal of proteome research Vol. 10; no. 7; pp. 3076 - 3088
• Main Authors: Zhang, Qibin, Monroe, Matthew E, Schepmoes, Athena A, Clauss, Therese R. W, Gritsenko, Marina A, Meng, Da, Petyuk, Vladislav A, Smith, Richard D, Metz, Thomas O
• Format: Journal Article
• Language: English
• Published: United States American Chemical Society 01.07.2011
• Subjects: 60 APPLIED LIFE SCIENCES; Amadori compound; Amino Acid Motifs; AMINO ACIDS; BASIC BIOLOGICAL SCIENCES; Biomarkers - blood; Biomarkers - chemistry; Blood Proteins - analysis; Blood Proteins - chemistry; boronate affinity chromatography; Chromatography, Affinity; Chromatography, High Pressure Liquid; Diabetes Complications - blood; Diabetes Complications - physiopathology; DIABETES MELLITUS; Diabetes Mellitus, Type 2 - blood; Diabetes Mellitus, Type 2 - physiopathology; electron transfer dissociation; Environmental Molecular Sciences Laboratory; erythrocyte; ERYTHROCYTES; Erythrocytes - chemistry; FRACTIONATION; Glycation End Products, Advanced - blood; Glycation End Products, Advanced - chemistry; glycation motif; Glycopeptides - blood; Glycopeptides - chemistry; Glycosylation; Humans; Hyperglycemia - blood; Hyperglycemia - physiopathology; Molecular Sequence Data; nonenzymatic glycation; Peptide Fragments - blood; Peptide Fragments - chemistry; PEPTIDES; PLASMA; Plasma - chemistry; PROTEINS; Proteomics - methods; red blood cell; Tandem Mass Spectrometry; Trypsin - metabolism; type 2 diabetes mellitus; boronate affinity chromatography; electron transfer dissociation; type 2 diabetes mellitus; Amadori compound; nonenzymatic glycation; plasma; glycation motif; erythrocyte; red blood cell

Nonenzymatic glycation of proteins sets the stage for formation of advanced glycation end-products and development of chronic complications of diabetes. In this report, we extended our previous methods on proteomics analysis of glycated proteins to comprehensively identify glycated proteins in contr...