In Vivo Detection of Phospholipase C by Enzyme-Activated Near-Infrared Probes

• Published in: Bioconjugate chemistry Vol. 22; no. 12; pp. 2434 - 2443
• Main Authors: Mawn, Theresa M, Popov, Anatoliy V, Beardsley, Nancy J, Stefflova, Klara, Milkevitch, Matthew, Zheng, Gang, Delikatny, E. James
• Format: Journal Article
• Language: English
• Published: United States American Chemical Society 21.12.2011
• Subjects: Animals; Cancer; Cell Line, Tumor; Enzyme Activation; Enzymes; Fluorescent Dyes - chemistry; Humans; Male; Medical imaging; Mice; Mice, Nude; Microscopy, Confocal; Phospholipids - chemistry; Prostatic Neoplasms - enzymology; Rodents; Signal transduction; Tumors; Type C Phospholipases - analysis; Type C Phospholipases - metabolism

In this article, the characterization of the first near-infrared (NIR) phospholipase-activated molecular beacon is reported, and its utility for in vivo cancer imaging is demonstrated. The probe consists of three elements: a phospholipid (PL) backbone to which the NIR fluorophore, pyropheophorbide a...