Antibody Repertoire Profiling Using Bacterial Display Identifies Reactivity Signatures of Celiac Disease

• Published in: Analytical chemistry (Washington) Vol. 85; no. 2; pp. 1215 - 1222
• Main Authors: Spatola, Bradley N, Murray, Joseph A, Kagnoff, Martin, Kaukinen, Katri, Daugherty, Patrick S
• Format: Journal Article
• Language: English
• Published: United States American Chemical Society 15.01.2013
• Subjects: Adult; antibodies; Antibodies - analysis; Antibodies - immunology; antibody specificity; Antigen-Antibody Reactions; Antigens; Autoimmune diseases; Bacteria; blood serum; Celiac disease; Celiac Disease - diagnosis; Celiac Disease - immunology; Cell Surface Display Techniques; Escherichia coli - chemistry; Escherichia coli - immunology; Female; Flow Cytometry; fluorescent dyes; gliadin; Humans; Male; Middle Aged; patients; peptide libraries; Peptide Library; Peptides; Peptides - chemistry; Peptides - immunology; protein-glutamine gamma-glutamyltransferase; Reagents; Sensitivity and Specificity; Tissues

A general strategy to identify serum antibody specificities associated with a given disease state and peptide reagents for their detection was developed using bacterial display peptide libraries and multiparameter flow cytometry (MPFC). Using sera from patients with celiac disease (CD) (n = 45) or h...