Quantifying Intracellular Nanoparticle Distributions with Three-Dimensional Super-Resolution Microscopy

• Published in: ACS nano Vol. 17; no. 9; pp. 8376 - 8392
• Main Authors: Sheth, Vinit, Chen, Xuxin, Mettenbrink, Evan M., Yang, Wen, Jones, Meredith A., M’Saad, Ons, Thomas, Abigail G., Newport, Rylee S., Francek, Emmy, Wang, Lin, Frickenstein, Alex N., Donahue, Nathan D., Holden, Alyssa, Mjema, Nathan F., Green, Dixy E., DeAngelis, Paul L., Bewersdorf, Joerg, Wilhelm, Stefan
• Format: Journal Article
• Language: English
• Published: United States American Chemical Society 09.05.2023
• Subjects: Animals; Imaging, Three-Dimensional; Mammals; Mass Spectrometry; Metal Nanoparticles - chemistry; Microscopy, Electron; Nanomedicine; nanomedicine; expansion microscopy; label-free; nanoparticles; 3D imaging; super-resolution

Super-resolution microscopy can transform our understanding of nanoparticle–cell interactions. Here, we established a super-resolution imaging technology to visualize nanoparticle distributions inside mammalian cells. The cells were exposed to metallic nanoparticles and then embedded within differen...