Contribution of Antibody Hydrodynamic Size to Vitreal Clearance Revealed through Rabbit Studies Using a Species-Matched Fab

• Published in: Molecular pharmaceutics Vol. 13; no. 9; pp. 2996 - 3003
• Main Authors: Shatz, Whitney, Hass, Philip E, Mathieu, Mary, Kim, Hok Seon, Leach, Kim, Zhou, Michelle, Crawford, Yongping, Shen, Amy, Wang, Kathryn, Chang, Debby P, Maia, Mauricio, Crowell, Susan R, Dickmann, Leslie, Scheer, Justin M, Kelley, Robert F
• Format: Journal Article
• Language: English
• Published: United States American Chemical Society 06.09.2016
• Subjects: Animals; Antibodies, Monoclonal - administration & dosage; Antibodies, Monoclonal - pharmacokinetics; CHO Cells; Cricetulus; Enzyme-Linked Immunosorbent Assay; Hydrodynamics; Immunoglobulin Fab Fragments - administration & dosage; Immunoglobulin Fab Fragments - metabolism; Intravitreal Injections; Kinetics; Polyethylene Glycols - chemistry; Rabbits; long acting delivery; ocular pharmacokinetics; PEGylation; hydrodynamic radius

We have developed a tool Fab fragment of a rabbit monoclonal antibody that is useful for early evaluation in rabbit models of technologies for long acting delivery (LAD) of proteins to the eye. Using this Fab we show that vitreal clearance can be slowed through increased hydrodynamic size. Fab (G10r...