Drug Distribution to Retinal Pigment Epithelium: Studies on Melanin Binding, Cellular Kinetics, and Single Photon Emission Computed Tomography/Computed Tomography Imaging

• Published in: Molecular pharmaceutics Vol. 13; no. 9; pp. 2977 - 2986
• Main Authors: Rimpelä, Anna-Kaisa, Schmitt, Mechthild, Latonen, Satu, Hagström, Marja, Antopolsky, Maxim, Manzanares, José A, Kidron, Heidi, Urtti, Arto
• Format: Journal Article
• Language: English
• Published: United States American Chemical Society 06.09.2016
• Subjects: Animals; Cells, Cultured; Chloroquine - metabolism; Eye - metabolism; Hydrogen-Ion Concentration; Iodine Radioisotopes; Kinetics; Melanins - metabolism; Methotrexate - metabolism; Nadolol - metabolism; Protein Binding; Rats; Retinal Pigment Epithelium - metabolism; Single Photon Emission Computed Tomography Computed Tomography - methods; Swine; Timolol - metabolism; melanin binding; ocular pharmacokinetics; retinal pigment epithelium; binding parameters; SPECT/CT

Melanin binding is known to affect the distribution and elimination of ocular drugs. The purpose of this study was to evaluate if the extent of drug uptake to primary retinal pigment epithelial (RPE) cells could be estimated based on in vitro binding studies with isolated melanin and evaluate the su...