Bioinspired l‑Proline Oligomers for the Cryopreservation of Oocytes via Controlling Ice Growth

• Published in: ACS applied materials & interfaces Vol. 12; no. 16; pp. 18352 - 18362
• Main Authors: Qin, Qingyuan, Zhao, Lishan, Liu, Zhang, Liu, Tao, Qu, Jiangxue, Zhang, Xiaowei, Li, Rong, Yan, Liying, Yan, Jie, Jin, Shenglin, Wang, Jianjun, Qiao, Jie
• Format: Journal Article
• Language: English
• Published: United States American Chemical Society 22.04.2020
• Subjects: Animals; Antifreeze Proteins - chemistry; Antifreeze Proteins - pharmacology; Cell Survival - drug effects; cryopreservation; Cryopreservation - methods; cryoprotectants; Cryoprotective Agents - chemistry; Cryoprotective Agents - pharmacology; crystals; dimethyl sulfoxide; Embryonic Development - drug effects; ethylene glycol; Female; freeze-thaw cycles; freezing; genetic engineering; glycoproteins; Ice; Male; Mice; mitochondria; Oligopeptides - chemistry; Oligopeptides - pharmacology; oocytes; Oocytes - cytology; Oocytes - drug effects; osmotic pressure; proline; Proline - chemistry; Proline - pharmacology; Reproductive Techniques, Assisted; surface area; survival rate; toxicity; variance; survival rate; antifreeze (glyco)proteins; ice recrystallization inhibition; mitochondrial function; cryopreservation of oocytes; L-proline oligomers

Various types of cells are routinely cryopreserved in modern regenerative and cell-based medicines. For instance, the oocyte is one of the most demanding cells to be cryopreserved in genetic engineering and human-assisted reproductive technology (ART). However, the usage of cryopreserved oocytes in...