Nongenetic Approach for Imaging Protein Dimerization by Aptamer Recognition and Proximity-Induced DNA Assembly

• Published in: Journal of the American Chemical Society Vol. 140; no. 12; pp. 4186 - 4190
• Main Authors: Liang, Hong, Chen, Shan, Li, Peipei, Wang, Liping, Li, Jingying, Li, Juan, Yang, Huang-Hao, Tan, Weihong
• Format: Journal Article
• Language: English
• Published: United States American Chemical Society 28.03.2018
• Subjects: dimerization; DNA assembly; image analysis; oligonucleotides; receptors; signal transduction; transforming growth factor beta

Herein, we report a nongenetic and real-time approach for imaging protein dimerization on living cell surfaces by aptamer recognition and proximity-induced DNA assembly. We use the aptamer specific for the receptor monomer as a recognition probe. When receptor dimerization occurs, the dimeric recept...