Quenched Ligand-Directed Tosylate Reagents for One-Step Construction of Turn-On Fluorescent Biosensors

• Published in: Journal of the American Chemical Society Vol. 131; no. 25; pp. 9046 - 9054
• Main Authors: Tsukiji, Shinya, Wang, Hangxiang, Miyagawa, Masayoshi, Tamura, Tomonori, Takaoka, Yousuke, Hamachi, Itaru
• Format: Journal Article
• Language: English
• Published: WASHINGTON American Chemical Society 01.07.2009; Amer Chemical Soc
• Subjects: Amino Acid Sequence; Biosensing Techniques - methods; Carbonic Anhydrase II - antagonists & inhibitors; Carbonic Anhydrase II - metabolism; Chemistry; Chemistry, Multidisciplinary; Enzyme Inhibitors - analysis; Enzyme Inhibitors - metabolism; Escherichia coli - chemistry; Escherichia coli - genetics; Fluorescent Dyes - chemical synthesis; Fluorescent Dyes - chemistry; Fluorescent Dyes - metabolism; Humans; Ligands; Models, Molecular; Phosphatidylinositol 3-Kinases - chemistry; Phosphatidylinositol 3-Kinases - genetics; Phosphatidylinositol 3-Kinases - metabolism; Phosphopeptides - analysis; Phosphopeptides - metabolism; Phosphotyrosine - analysis; Phosphotyrosine - metabolism; Physical Sciences; Protein Binding; Protein Structure, Tertiary; Science & Technology; Tosyl Compounds - chemical synthesis; Tosyl Compounds - chemistry; Tosyl Compounds - metabolism; INOSITOL 1,4,5-TRISPHOSPHATE; PHOSPHORYLATED PEPTIDE; MOLECULAR RECOGNITION; DRUG DISCOVERY; IN-VIVO; PHOTOAFFINITY-LABELING MODIFICATION; SACCHARIDE-BIOSENSORS; CARBONIC-ANHYDRASE INHIBITORS; AQUEOUS-SOLUTION; LIVING CELLS

Semisynthetic fluorescent biosensors consisting of a protein framework and a synthetic fluorophore are powerful analytical tools for specific detection of biologically relevant molecules. We report herein a novel method that allows for the construction of turn-on fluorescent semisynthetic biosensors...