Photochromic Fluorescent Probe Strategy for the Super-resolution Imaging of Biologically Important Biomarkers

• Published in: Journal of the American Chemical Society Vol. 142; no. 42; pp. 18005 - 18013
• Main Authors: Chai, Xianzhi, Han, Hai-Hao, Sedgwick, Adam C, Li, Na, Zang, Yi, James, Tony D, Zhang, Junji, Hu, Xi-Le, Yu, Yang, Li, Yao, Wang, Yan, Li, Jia, He, Xiao-Peng, Tian, He
• Format: Journal Article
• Language: English
• Published: WASHINGTON American Chemical Society 21.10.2020; Amer Chemical Soc
• Subjects: beta-Galactosidase - analysis; beta-Galactosidase - metabolism; biomarkers; Biomarkers - analysis; Biomarkers - metabolism; Cell Line; Chemistry; Chemistry, Multidisciplinary; fluorescence; fluorescent dyes; Fluorescent Dyes - chemical synthesis; Fluorescent Dyes - chemistry; galactose; human serum albumin; Humans; hybrids; microscopy; Microscopy, Confocal; Molecular Structure; Optical Imaging; permeability; Photochemical Processes; photoisomerization; photostability; phototoxicity; Physical Sciences; Science & Technology; Serum Albumin, Human - chemistry; solubility; CELLS; NANOPARTICLES; FLUOROPHORES; BETA-GALACTOSIDASE; POSTTRANSLATIONAL MODIFICATIONS; SENESCENCE; IONIZING-RADIATION; INTRAMOLECULAR SPIROCYCLIZATION

Here, we report a β-galactosidase (β-Gal)-responsive photochromic fluorescent probe, NpG, that was designed to prebind to human serum albumin (HSA) to form the probe/protein hybrid, NpG@HSA. The formation of NpG@HSA led to an increase in fluorescence emission (520 nm) corresponding to the binding of...