Identification and Characterization of a Cryptic Bifunctional Type I Diterpene Synthase Involved in Talaronoid Biosynthesis from a Marine-Derived Fungus

We report the identification of the tnd biosynthetic cluster from the marine-derived fungus Aspergillus flavipes and the in vivo characterization of a cryptic type I diterpene synthase. The heterologous expression of the bifunctional terpene synthase led to the discovery of a diterpene backbone, tal...

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Published inOrganic letters Vol. 24; no. 38; pp. 7037 - 7041
Main Authors Zhang, Peng, Wu, Guangwei, Heard, Stephanie C., Niu, Changshan, Bell, Stephen A., Li, Fengli, Ye, Ying, Zhang, Yonghui, Winter, Jaclyn M.
Format Journal Article
LanguageEnglish
Published American Chemical Society 30.09.2022
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Letter
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Summary:We report the identification of the tnd biosynthetic cluster from the marine-derived fungus Aspergillus flavipes and the in vivo characterization of a cryptic type I diterpene synthase. The heterologous expression of the bifunctional terpene synthase led to the discovery of a diterpene backbone, talarodiene, harboring a benzo­[a]­cyclopenta­[d]­cyclooctane tricyclic fused ring system. The conversion of geranylgeranyl diphosphate to talarodiene was investigated using 13C-labeling studies, and stable isotope tracer experiments showed the biotransformation of talarodiene into talaronoid C.
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ISSN:1523-7060
1523-7052
DOI:10.1021/acs.orglett.2c02904