Whole-Proteome Peptide Microarrays for Profiling Autoantibody Repertoires within Multiple Sclerosis and Narcolepsy

• Published in: Journal of proteome research Vol. 16; no. 3; pp. 1300 - 1314
• Main Authors: Zandian, Arash, Forsström, Björn, Häggmark-Månberg, Anna, Schwenk, Jochen M, Uhlén, Mathias, Nilsson, Peter, Ayoglu, Burcu
• Format: Journal Article
• Language: English
• Published: United States American Chemical Society 03.03.2017
• Subjects: adaptive immunity; Adolescent; Adult; Aged; amino acids; autoantibodies; Autoantibodies - blood; autoantibody profiling; autoantigens; Autoantigens - blood; autoimmune diseases; blood serum; Cell Adhesion Molecules, Neuronal - blood; Child; disease diagnosis; epitope mapping narcolepsy; epitopes; Female; human diseases; Humans; immunoglobulin G; Male; MAP Kinase Kinase Kinases - blood; microarray technology; Middle Aged; multiple sclerosis; Multiple Sclerosis - blood; narcolepsy; Narcolepsy - blood; Nerve Tissue Proteins - blood; peptide microarrays; peptides; Protein Array Analysis - methods; proteome; Proteome - analysis; sclerosis; Young Adult; autoantibody profiling; peptide microarrays; narcolepsy; multiple sclerosis; epitope mapping

The underlying molecular mechanisms of autoimmune diseases are poorly understood. To unravel the autoimmune processes across diseases, comprehensive and unbiased analyses of proteins targets recognized by the adaptive immune system are needed. Here we present an approach starting from high-density p...