Quantitative Online Liquid Chromatography–Surface-Enhanced Raman Scattering (LC-SERS) of Methotrexate and its Major Metabolites

• Published in: Analytical chemistry (Washington) Vol. 89; no. 12; pp. 6702 - 6709
• Main Authors: Subaihi, Abdu, Trivedi, Drupad K, Hollywood, Katherine A, Bluett, James, Xu, Yun, Muhamadali, Howbeer, Ellis, David I, Goodacre, Royston
• Format: Journal Article
• Language: English
• Published: United States American Chemical Society 20.06.2017
• Subjects: Analytical chemistry; Chemistry; Chromatography; Chromatography, Liquid; Drugs; Elution; Humans; Internet; Liquid chromatography; Mass spectrometry; Mass spectroscopy; Metabolites; Methotrexate; Methotrexate - analogs & derivatives; Methotrexate - metabolism; Methotrexate - urine; Molecular Structure; Patients; Raman spectra; Raman spectroscopy; Real time; Scattering; Separation; Spectroscopy; Spectrum Analysis, Raman - methods; Spikes; Surface Properties; Urine; Water analysis

The application of Raman spectroscopy as a detection method coupled with liquid chromatography (LC) has recently attracted considerable interest, although this has currently been limited to isocratic elution. The combination of LC with rapidly advancing Raman techniques, such as surface-enhanced Ram...