Genetic identification and purification of the respiratory NADH dehydrogenase of Escherichia coli

• Published in: Biochemistry (Easton) Vol. 20; no. 7; pp. 2041 - 2047
• Main Authors: Jaworowski, Anthony, Campbell, Hugh D, Poulis, Maria I, Young, Ian G
• Format: Journal Article
• Language: English
• Published: United States American Chemical Society 31.03.1981
• Subjects: Cell Membrane - enzymology; Escherichia coli - enzymology; Genes; Genetic Complementation Test; Molecular Weight; Mutation; NAD(P)H Dehydrogenase (Quinone); NADH, NADPH Oxidoreductases - genetics; Plasmids; Quinone Reductases - genetics; Quinone Reductases - isolation & purification

Escherichia coli membrane particles were solubilized with potassium cholate. An NADH:ubiquinone oxidoreductase was resolved by hydroxylapatite chromatography of the solubilized material. This enzyme has been identified as the respiratory NADH dehydrogenase since it is absent in chromatograms of solu...