Site-Specific Covalent Labeling of DNA Substrates by an RNA Transglycosylase

• Published in: Journal of the American Chemical Society Vol. 145; no. 14; pp. 8099 - 8106
• Main Authors: Tota, Ember M., Devaraj, Neal K.
• Format: Journal Article
• Language: English
• Published: United States American Chemical Society 12.04.2023
• Subjects: DNA; Escherichia coli; Guanine; RNA - chemistry; RNA, Transfer

Bacterial tRNA guanine transglycosylases (TGTs) catalyze the exchange of guanine for the 7-deazaguanine queuine precursor, prequeuosine1 (preQ1). While the native nucleic acid substrate for bacterial TGTs is the anticodon loop of queuine-cognate tRNAs, the minimum recognition sequence for the enzyme...