Loss of the E6AP Ubiquitin Ligase Induces p53-Dependent Phosphorylation of Human Papillomavirus 18 E6 in Cells Derived from Cervical Cancer

• Published in: Journal of virology Vol. 96; no. 6; p. e0150321
• Main Authors: Vats, Arushi, Skrabar, Neva, Del Sal, Giannino, Banks, Lawrence
• Format: Journal Article
• Language: English
• Published: United States American Society for Microbiology 23.03.2022
• Subjects: Cell Line, Tumor; Female; Human papillomavirus 18 - metabolism; Humans; Phosphorylation; Tumor Suppressor Protein p53 - metabolism; Ubiquitin-Protein Ligases - genetics; Ubiquitin-Protein Ligases - metabolism; Uterine Cervical Neoplasms - physiopathology; Uterine Cervical Neoplasms - virology; Virology; Virus-Cell Interactions; E6AP; E6; PBM; DNA PK; HPV; p53

This study demonstrates that the knockdown of E6AP from cervical cancer-derived cells leads to an increase in phosphorylation of the E6 oncoprotein. We show that this phosphorylation of E6 requires p53 transcriptional activity and the enzyme DNA PK. This study therefore defines a feedback loop where...