Chirality Dependent Potency Enhancement and Structural Impact of Glycol Nucleic Acid Modification on siRNA

• Published in: Journal of the American Chemical Society Vol. 139; no. 25; pp. 8537 - 8546
• Main Authors: Schlegel, Mark K, Foster, Donald J, Kel’in, Alexander V, Zlatev, Ivan, Bisbe, Anna, Jayaraman, Muthusamy, Lackey, Jeremy G, Rajeev, Kallanthottathil G, Charissé, Klaus, Harp, Joel, Pallan, Pradeep S, Maier, Martin A, Egli, Martin, Manoharan, Muthiah
• Format: Journal Article
• Language: English
• Published: WASHINGTON American Chemical Society 28.06.2017; Amer Chemical Soc; American Chemical Society (ACS)
• Subjects: Chemistry; Chemistry, Multidisciplinary; Physical Sciences; Science & Technology; SYSTEM; IN-VITRO; DUPLEX STRUCTURE; ANALOGS; STABILITY; CRYSTALLOGRAPHY; RNA INTERFERENCE ACTIVITY; CLEAVAGE; MODULATION; ARGONAUTE2

Here we report the investigation of glycol nucleic acid (GNA), an acyclic nucleic acid analogue, as a modification of siRNA duplexes. We evaluated the impact of (S)- or (R)-GNA nucleotide incorporation on RNA duplex structure by determining three individual crystal structures. These structures indic...